Acoustofluidic DNA Diagnosis Chip (ADD-Health) Autofinanziato (DIT.AD010.046)

Thematic area

Engineering, ICT and technologies for energy and transportation

Project area

Structure responsible for the research project

Institute of applied physics "Nello Carrara" (IFAC)

Project manager

FRANCESCO BALDINI
Phone number: 055 5226323
Email: f.baldini@ifac.cnr.it

Abstract

The "Acoustofluidic DNA Diagnosis Chip" project aims to develop a novel biomedical device for personalized medicine, which enables the extraction and detection of somatic mutations in EGFR directly from the whole blood. The device relies on the combination of a module for cell-free DNA extraction and purification (DNA-Chip) and on a module for DNA amplification and quantification (PCR-Chip). The DNA-chip (developed by the Chinese party) is a microfluidic chip, which integrates functionalized microbeads with bulk acoustic wave devices to achieve plasma separation and efficient DNA extraction. The PCR-chip (developed by the Italian party) amplifies and detects the cell-free DNA extracted in the DNA-chip. In order to ensure high specificity, fluorescence-based real-time polymerase chain reaction (PCR) will be implemented. The on-chip PCR module is based on the integration, on a single glass substrate, of thin film heaters and amorphous silicon temperature and light sensors, in order to ensure high sensitivity and miniaturized systems. The integration of the two modules into a portable and miniaturized system leads to an innovative lab-on-chip for molecular diagnosis featuring low cost

Start date of activity

01/01/2020

Keywords

lab-on-chip, DNA analysis, acoustofluidic

Last update: 08/10/2024