Structural insights into the Cdc45 replication factor
Il 25/03/2019 ore 14.30 - 15.30
Sala Conferenze Cnr, Area della Ricerca Napoli1, via Pietro Castellino 111, 80131 Napoli
Silvia Onesti, from the Elettra – Sincrotrone Trieste S.C.p.A., Trieste, Italy, will give a Seminar on 'Structural insights into the Cdc45 replication factor'.
The Structural Biology Laboratory at Elettra applies molecular and structural biology tools (from macromolecular crystallography, to small angle X-ray scattering to cryo-electron microscopy) to study the basic genetic processes within the cell and to characterise some of the proteins and macromolecular complexes involved. Our research focus on DNA replication and DNA repair. These are crucial events in the cell cycle, underpinning cellular processes with important consequences such as cell proliferation and genome stability. Failure to control these processes causes chromosome instability, which can lead to the development of cellular abnormalities, genetic diseases and the onset of cancer. Cdc45 is an essential replication factor in eukaryotes, as - together with GINS - is essential for the activation and progression of the MCM replicative helicase. The eukaryotic Cdc45 proteins show a weak but significant relationship to a large family of exonucleases that has been described as RecJ/DHH family. We have determined the crystal structures of two archaeal homologues of Cdc45, and have characterised their enzymatic activity towards both DNA and RNA substrates, alone and in the presence of the MCM and GINS counterparts. We have obtained the atomic structures with and without nucleic acid substrates, revealing a mode of binding which is very different from the bacterial RecJ counterparts.
Organizzato da:
Cnr-Ibp
Referente organizzativo:
Francesca M. Pisani
CNR - Istituto di biochimica delle proteine
Via Pietro Castellino 111, 80131 Napoli
fm.pisani@ibp.cnr.it
Modalità di accesso: ingresso libero
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