PRIN 2017 - 2017FJCPEX - Alberto Bossi (DCM.AD006.157)

Area tematica

Scienze chimiche e tecnologie dei materiali

Area progettuale

Struttura responsabile del progetto di ricerca

Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC)

Responsabile di progetto

ALBERTO BOSSI
Telefono: 0250995627
E-mail: alberto.bossi@istm.cnr.it

Abstract

The unique chemical, morphological, opto-electrical characteristics of 3D mesostructured functional materials, state of the art ultrabright Ir luminophores and the high analytical performance of
electrochemiluminescence techniques (ECL) will serve to 3D-FARE to develop novel analytical platforms for (i) highly sensitive functional imaging of living/fixed cells (in particular of circulating tumor cells) and (ii) for the detection of tumor associated antigens (TAA) in human blood. Heteroleptic cyclometalated Ir complexes, either charged or neutral, with complementary emissions in the visible region (eg.
bluish-green) or in the NIR and suitable functional groups and linker chains for the anchoring purposes (both to antibodies and surfaces) will be selected and prepared in view of enhanced biosensing ECL
emission.

Obiettivi

The aim of 3D-FARE is to demonstrate a proof of principle for a novel platform for the highly sensitive detection of cancer cells and biomarkers. The system merges complementary expertises where
ultrabright Ir luminophores are associated to the 3D-FARE electrodes containing open porous structures and concur to the generation of intense electrogenerated chemiluminescence (ECL). ECL is produced
according to the coreactant approach where an excited state is generated through the reaction between the fluorophore and the co-reactant, both oxidised at the electrode. Scientifically, the proposed strategy
rests on three pillars:
(i) tailored design of 3D mesoporous electrodes suited for the interaction with cells and biomolecules and their fabrication according to either top-down or bottom-up strategies;
(ii) development of novel ultrabright Ir organometallic luminophores suited for the bioconjugation; (iii) assembly of an analytical platform for highly sensitive ECL imaging of cells and detection of biomarkers.

Data inizio attività

07/03/2019

Parole chiave

electrochemiluminiscence, organometallics, biosensors

Ultimo aggiornamento: 06/06/2025