ADOR-SOLE (DCM.AD007.219)
Thematic area
Chemical sciences and materials technology
Project area
Chimica e materiali per la salute e le scienze della vita (DCM.AD007)Structure responsible for the research project
Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC)
Project manager
FRANCESCO DAMIN
Phone number: 0228500042
Email: francesco.damin@scitec.cnr.it
Abstract
In the field of molecular diagnostics for infectious diseases, many biotechnology companies are trying to develop diagnostic platforms to give clear results in a short time and in multiplexing mode. In this context, Ador has developed a new innovative technology (NATlab) based on the polymerase chain reaction (PCR) process as well as an isothermal method called rolling circle amplification (RCA), to enable NATlab to increase the speed of providing sample-to-answer diagnostics to under 30 minutes. The NATlab can analyze several pathogens (currently up to 400) at the same time thus enabling it to detect all the possible pathogens associated with diseases such as meningitis, sepsis and respiratory diseases. The NATlab system consists of a benchtop stackable or daisy chained analyzer and disposable cartridges. The patented cartridges contain a carbon array blotted with hydrogel, which is where the testing of a DNA or RNA sample - whether in blood, urine or any other bodily liquid - occurs. Ador is now in the advanced stages of incorporating RCA in the carbon array and is working to complete the development of a full system based on this process
Goals
Following the protocol provided by Ador, the Institute will blot carbon arrays, each containing 100 blots of Hydrogel using the Scienion spotter available at CNR. All chemicals, nozzles and carbon array substrates will be provided by Ador.
Institute will deliver 400 carbon arrays, each containing 100 blots of hydrogel, per calendar month (three spotting sessions per calendar month) for the duration of the agreement.
In addition, the spotting conditions such as humidity and the residence time in the spotter will be studied and the morphology of the spots will be optimized using different types of additives.
Start date of activity
18/06/2021
Keywords
spotting, hydrogel, carbon array
Last update: 08/05/2024