AIRC 2019 Nano-patterned metastatic melanoma for quantifying metabolic changes in mediated drug resistance (DFM.AD004.254)

Thematic area

Physical sciences and technologies of matter

Project area

Structure responsible for the research project

Institute of Nanotechnology (NANOTEC)

Project manager

LORETTA LAUREANA DEL MERCATO
Phone number: 0832319825
Email: loretta.delmercato@nanotec.cnr.it

Abstract

The goal of this project is to recreate in vitro, as closely as possible, the features of the melanoma tumor in the proper microenvironmental context and to use these 3D in vitro models of metastatic melanoma to characterize the dynamics of phenotypic plasticity of primary tumors, biopsies or cell lines derived from cancer patients thus facilitating risk stratification of cancer patients and developing personalized treatment strategies. I will generate 3D in vitro models of metastatic melanoma that are capable of 'mapping' extracellular gradients of lactate and O2 with submicron spatial resolution, to establish whether direct quantitative readouts of these gradients relate to biologically-relevant changes in cell response to individual therapies, going beyond the possibilities offered by current standard chemosensitivity assays.

Goals

MELANOMA; GLUCOSE METABOLISM AND/OR WARBURG EFFECT; IN VITRO IMAGING AND/OR LIVE CELL IMAGING; DRUG RESPONSE AND/OR RESISTANCE

Start date of activity

02/01/2020

Keywords

BIOSENSORS, 3D TUMOR MODELS, IMAGING

Last update: 11/09/2024