HOTSOLUTE (DCM.AD004.071)

Thematic area

Chemical sciences and materials technology

Project area

Structure responsible for the research project

Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC)

Project manager

DANIELA MONTI
Phone number: 0228500025
Email: daniela.monti@icrm.cnr.it

Abstract

This ERA-CoBiotech project is developing thermophilic in vitro enzyme cascades as well as two new chassis, the thermophilic bacterium Thermus thermophilus (Tth, 65-75°C, pH 7.0) and the thermoacidophilic archaeon Sulfolobus acidocaldarius (Saci, 75-80°C, pH 2-4), as new thermophilic, bacterial and archaeal platforms for the in vivo production of novel high added-value products. These include 'extremolytes' that are small molecular compatible solutes found naturally in the cells of thermophilic species. Extremolytes possess great potential for application in the food, health care consumer care and cosmetics market, but cannot be produced in current mesophilic production hosts. Specifically, the development of the newly designed 'cell factories' and cascades will be used for the production of three extremolytes, cyclic 2,3 di-phosphoglycerate (cDPG), di-myo-1,1'-inositol-phosphate (DIP) and mannosylglycerate (MG).

Start date of activity

01/03/2018

Keywords

extremolytes, enzyme cascades, cell factories

Last update: 05/06/2025