SERVIZIO SEQUENZIAMENTO PROTEINE (DBA.AD006.017)

Thematic area

Biology, agriculture and food sciences

Project area

Structure responsible for the research project

Institute of plant genetics (IBBR)

Project manager

GABRIELLAKATALIN POCSFALVI
Phone number: 816132570
Email: gabriella.pocsfalvi@ibbr.cnr.it

Abstract

We offer N-terminal protein/peptide sequencing using a Shimadzu PPSQ 33B sequencer at IBBR-Napoli. The method is based on the Edman degradation reaction, which labels the N-terminal amino acid residues within a polypeptide such that they can be cleaved from the chain in a stepwise manner. The benefits of using Edman degradation for protein sequencing are:
o Guaranteed N-terminal sequence of proteins;
o No sample pre-treatment required:
o Differentiation of isobaric amino acids (leucine and isoleucine):
o Sequencing of unknown proteins not registered in databases.
In this project, we will extend the service of the facility (http://ibbr.cnr.it/ibbr/facilities/protein-peptide-sequencing/?lang=it) to third parties interested in.

Start date of activity

16/10/2018

Keywords

proteine, peptidi

Last update: 04/08/2025