INDEX - H2020 Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes (DCM.AD007.017)
Thematic area
Chemical sciences and materials technology
Project area
Chimica e materiali per la salute e le scienze della vita (DCM.AD007)Structure responsible for the research project
Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC)
Project manager
MARCELLA CHIARI
Phone number: 0228500035
Email: marcella.chiari@icrm.cnr.it
Abstract
The aim of Project INDEX is to isolate and characterize nanoparticles available in bodily fluids through development and integration of novel technological breakthroughs. The technology will enable the analysis of clinically valuable nanoparticles called exosomes towards new generation diagnostics. Exosomes are known to mediate communication between cells and their effective utilization holds a great promise of revolutionizing the standard of clinical care. However, their detection and molecular profiling is technically challenging. The proposed technology will isolate exosomes that are as small as 30nm in diameter from human plasma with high purity, and provide in-depth, multi-parameter characterization of the particles through digital counting, size determination, and biological phenotyping.
Goals
Towards this goal: (1) Novel microfluidics will be developed and used for efficient magnetic enrichment; (2) Isolated particles will be detected and analyzed with a novel biological nanoparticle (BNP) sensor (3) Immune-capture and release chemistries as well as phenotyping assays will be developed; (4) Critically, complete on-chip integration of isolation, detection and analysis will be accomplished; (5) Utility of in-depth exosome characterization will be demonstrated with clinical samples for lung cancer.
Start date of activity
01/10/2017
Keywords
Digital detection, exosomes, interferometry
Last update: 20/09/2024