PROGETTO FELLOWSHIP VITALE N. 009_FC_2016 TITOLO: DISSECTION OF T CELL MEDIATED IMMUNITY TO GLUTEN IN DIFFFERENT PHASES OF CELIAC DISEASE. (DSB.AD006.182)
Thematic area
Project area
Biologia Molecolare/Cellulare (DSB.AD006)Structure responsible for the research project
Istituto di Biochimica e Biologia Cellulare (IBBC)
Project manager
CARMELA GIANFRANI
Phone number: 0816132224
Email: c.gianfrani@ibp.cnr.it
Abstract
PROGETTO FELLOWSHIP VITALE N. 009_FC_2016 TITOLO: DISSECTION OF T CELL MEDIATED IMMUNITY TO GLUTEN IN DIFFFERENT PHASES OF CELIAC DISEASE.
Celiac disease (CD) is an immune mediated disorder elicited by the ingestion of wheat gluten and related prolamins that cause intestinal lesions. CD affects approximately 1-3% of the population and is strongly associated with HLA-Class II genes and presents different forms of enteropathy. The classical CD is characterized by atrophy of small intestinal villous. However, some subjects having the risk HLA genes and serological markers of CD but with a normal mucosal architecture are considered at high risk for developing the overt CD and are called potential CD patients. The intestinal mucosa of potential CD is infiltrated by both inflammatory and regulatory T cells, these latter most likely involved in preventing the villous atrophy. Exploiting the availability of these two groups of children (potential and acute celiacs), could give important information to understand the sequence of events takes place in the intestinal mucosa at the different stages of disease.
Goals
PROGETTO FELLOWSHIP VITALE N. 009_FC_2016 TITOLO: DISSECTION OF T CELL MEDIATED IMMUNITY TO GLUTEN IN DIFFFERENT PHASES OF CELIAC DISEASE.
The aim of the present proposal is to investigate the role of various T cell subtypes (i.e.Th1, Th2, Th17, Treg, Tr1) in both peripheral blood and small intestinal biopsies of children in different stages of CD, as in potential and acute CD. In particular, the phenotypical and functional features of gluten-reactive T cell lines will be assessed to compare the frequency of the T cell subsets infiltrating the mucosa in the disorder phases. Furthermore, dominant gluten epitopes, as well as TcR repertoire, will be identified and the expansion of specific TcR family will be correlated with immunogenic peptides, in each recruited subject. The analysis on T cell-mediated immunity to gluten in such special groups of young patients, could help in understanding the sequence of the immunological events at the base of CD pathogenesis.
Start date of activity
01/03/2017
Keywords
FELLOWSHIP, CELIAC, GLUTEN
Last update: 17/04/2024