Role of miR-200c in dystrophic muscle regeneration of mdx mice and DMD patients (DSB.AD006.341)

Area tematica

Scienze biomediche

Area progettuale

Struttura responsabile del progetto di ricerca

Istituto di Biochimica e Biologia Cellulare (IBBC)

Responsabile di progetto

FRANCESCA DESANTA
Telefono: 0690091
E-mail: francesca.desanta@cnr.it

Abstract

Duchenne Muscular Dystrophy (DMD) is one of the most common and severe dystrophies and it is associated with muscle degeneration, oxidative stress and chronic inflammation. Recent findings demonstrated that the micro-RNA miR-200c is the member of miR-200 family most involved in reactive oxygen species (ROS) production, inflammation and apoptosis. Moreover, we recently published a paper reporting the role of miR-200c in myogenic differentiation of myoblast cell line and we also showed higher level of miR-200c in dystrophic muscles of mdx mice, the most used mouse model for DMD, compared to wt mice. Preliminary analysis also demonstrated that miR-200c is significantly induced in in vitro differentiated myoblasts derived from DMD patients compared to differentiated myoblasts from healthy donors (D'Agostino, Torcinaro et al., 2018). The main objective of this project is to investigate the role of miR-200c in regeneration of skeletal muscles of mdx mice compared to wild type mice, focusing on the analysis of regenerative process, apoptosis, ROS and NO production. The expression pattern of miR-200c will be analyzed in whole muscles and satellite cells of mdx mice compared to wt mice. To

Data inizio attività

22/10/2021

Parole chiave

Skeletal muscle regeneration - Duchenne muscular Dystrophy (DMD), miR-200c, Oxidative stress

Ultimo aggiornamento: 08/06/2025