SERVIZIO SEQUENZIAMENTO PROTEINE (DBA.AD006.017)

Area tematica

Scienze bio-agroalimentari

Area progettuale

Struttura responsabile del progetto di ricerca

Istituto di Bioscienze e Biorisorse (IBBR)

Responsabile di progetto

GABRIELLAKATALIN POCSFALVI
Telefono: 816132570
E-mail: gabriella.pocsfalvi@ibbr.cnr.it

Abstract

We offer N-terminal protein/peptide sequencing using a Shimadzu PPSQ 33B sequencer at IBBR-Napoli. The method is based on the Edman degradation reaction, which labels the N-terminal amino acid residues within a polypeptide such that they can be cleaved from the chain in a stepwise manner. The benefits of using Edman degradation for protein sequencing are:
o Guaranteed N-terminal sequence of proteins;
o No sample pre-treatment required:
o Differentiation of isobaric amino acids (leucine and isoleucine):
o Sequencing of unknown proteins not registered in databases.
In this project, we will extend the service of the facility (http://ibbr.cnr.it/ibbr/facilities/protein-peptide-sequencing/?lang=it) to third parties interested in.

Data inizio attività

16/10/2018

Parole chiave

proteine, peptidi

Ultimo aggiornamento: 19/06/2025