Replication of a complex viral DNA element by eukaryotic proteins
Il 24/07/2025 ore 14.30 - 16.00
Via Pietro Castellino n. 111 Napoli c/o Area di Ricerca Napoli 1
Il seminario sarà tenuto tenuto dalla Dr.ssa Irene Gallina, Dipartimento di Medicina Molecolare - Università di Padova.
Herpesviruses are characterized by highly GC-rich genomes and several lines of evidence suggest the formation of G-quadruplexes (G4s) within these viral DNA sequences. Kaposis’s sarcoma associated herpesvirus (KSHV), the etiological agent of multiple lymphoproliferative diseases, contains an extensive non-coding repetitive region named terminal repeats (TRs) with a GC content higher than 80%. TRs are crucial for KSHV latency, mediating efficient latent viral genome replication and segregation during host cell division through binding of the essential latency-associated nuclear antigen (LANA). TRs have been previously proposed to form G4s. However, the function of these difficult-to-replicate regions within the genome of KSHV and how they are processed by the eukaryotic host replication machinery during viral latency remain unknown.
We exploit in vitro replication in Xenopus laevis egg extracts combined with work in infected human lymphoblasts as complementary es to dissect the contribution of DNA secondary structures and of LANA to the replication of TRs. By using mass-spectrometry based approaches, we also identify eukaryotic proteins and pathways recruited to the TRs and we dissect their contribution during DNA replication.
Organizzato da:
Istituto di Biochimica e Biologia cellulare
Referente organizzativo:
Ferdinando Febbraio
CNR - Istituto di Biochimica e Biologia Cellulare
ferdinando.febbraio@cnr.it
Modalità di accesso: ingresso libero
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