@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA425 pubblicazioni:autoreCNRDi prodotto:ID51465 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS083 prodottidellaricerca:prodotto prodotto:ID51465 . @prefix modulo: . modulo:ID5789 prodottidellaricerca:prodotto prodotto:ID51465 . modulo:ID2204 prodottidellaricerca:prodotto prodotto:ID51465 . @prefix rdf: . @prefix retescientifica: . prodotto:ID51465 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID51465 rdfs:label "Inactivation of Helicobacter pylori cagA gene affects motility. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID51465 pubblicazioni:anno "2004-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID51465 skos:altLabel "
Figura N, Trabalzini L, Mini R, Bernardini G, Scaloni A, Talamo F, Lusini P, Ferro E, Martelli P, Santucci A. (2004)
Inactivation of Helicobacter pylori cagA gene affects motility.
in Helicobacter (Camb. Mass.)
"^^rdf:HTML ; pubblicazioni:autori "Figura N, Trabalzini L, Mini R, Bernardini G, Scaloni A, Talamo F, Lusini P, Ferro E, Martelli P, Santucci A."^^xsd:string ; pubblicazioni:paginaInizio "185"^^xsd:string ; pubblicazioni:paginaFine "193"^^xsd:string ; pubblicazioni:numeroVolume "9(3)"^^xsd:string . @prefix ns11: . prodotto:ID51465 pubblicazioni:rivista ns11:ID199161 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "Inactivation of Helicobacter pylori cagA gene affects motility."^^xsd:string ; prodottidellaricerca:abstract "The cytotoxin-associated protein CagA is a Helicobacter pylori immunodominant antigen whose gene resides in the cag pathogenicity island. Our purpose was to determine if the disruption or deletion of cagA gene could have an effect on the expression of other proteins at the proteome level. We analyzed two H. pylori strains, 328 and G27 wild-type, bearing the cag pathogenicity island, and their respective isogenic cagA(-) mutants. METHODS: The proteomes of two H. pylori strains (328 and its isogenic mutant SPM328_DeltacagA) were resolved by two-dimensional electrophoresis and the digitalized images obtained were analysed both quantitatively and qualitatively. Peculiar spots of each strain were identified by mass spectrometry or by Western blotting. RESULTS: The comparison between the proteome expression of an H. pylori cagA(+) strain and an isogenic mutant strain where the cagA gene was disrupted showed that, as well as the lack of expression of CagA, both flagellin A and flagellin B expressions were significantly decreased. The cagA(-) isogenic mutant was nonmotile. G27_DeltacagA, in which CagA was inactivated by gene deletion, was nonmotile as well respecting to motile G27 wild-type strain. Moreover, reintroduction of cagA in G27_DeltacagA restored motility. CONCLUSIONS: Our results suggest that CagA could quantitatively influence flaA and flaB transcription or their subsequent translation and/or correct folding." ; prodottidellaricerca:prodottoDi modulo:ID5789 , istituto:CDS083 , modulo:ID2204 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA425 . ns11:ID199161 pubblicazioni:rivistaDi prodotto:ID51465 .