LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jchromb.2011.01.029 (literal)

Alternative label

• Lattanzio, Veronica Maria Teresa; Solfrizzo, M.; De Girolamo, Annalisa De; Chulze, Sofía Noemí; Torres, Adriana M.; Visconti, Angelo (2011)
  LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat
  in Journal of chromatography. B (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lattanzio, Veronica Maria Teresa; Solfrizzo, M.; De Girolamo, Annalisa De; Chulze, Sofía Noemí; Torres, Adriana M.; Visconti, Angelo (literal)

Pagina inizio

• 707 (literal)

Pagina fine

• 715 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.scopus.com/record/display.url?eid=2-s2.0-79952898756&origin=inward (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 879 (literal)

Rivista

• Journal of chromatography. B (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11-12 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Scienze delle Produzioni Alimentari; Universidad Nacional de Rio Cuarto (literal)

Titolo

• LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat (literal)

Abstract

• The understanding of mycotoxins transfer to biological fluids is challenged by the difficulties in performing and replicating in vivo experiments as well as the lack of suitable methods of analysis to detect simultaneously a range of chemically different metabolites at trace levels. LC-MS/MS has been used herein to study the urinary excretion profile of the mycotoxin deoxynivalenol in human and Wistar rat. Deoxynivalenol and deoxynivalenol glucuronide were found in both human and rat urines, whereas de-epoxydeoxynivalenol and its glucuronide conjugate were only detected in rat urine. The presence of two deoxynivalenol glucuronide isomers in Wistar rat urine has been shown for the first time. Structure confirmation of the detected metabolites was provided by the analysis of fragmentation patterns. A solid phase extraction clean up procedure allowing recoveries in the range 72-102% for deoxynivalenol, de-epoxydeoxynivalenol, and their glucuronide conjugates was optimized. A multiple reaction monitoring method for the simultaneous determination of all investigated metabolites was elaborated allowing the direct detection of deoxynivalenol metabolites without the hydrolysis step. Deoxynivalenol urinary levels in the range 0.003-0.008. ?g/ml were detected in healthy human subjects, whereas deoxynivalenol and de-epoxynivalenol levels between 1.9-4.9. ?g/ml and 1.6-5.9. ?g/ml, respectively were found in administered rat urine. These findings emphasize the relevance of the highly selective and sensitive LC-MS/MS technique for the direct detection and characterization of deoxynivalenol metabolites in complex biological matrices. © 2011 Elsevier B.V. (literal)

Prodotto di

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Autore CNR

• ANGELO VISCONTI (Unità di personale interno)
• VERONICA MARIA TERESA LATTANZIO (Unità di personale interno)
• ANNALISA DE GIROLAMO (Unità di personale interno)
• MICHELE SOLFRIZZO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELO VISCONTI (Unità di personale interno)
• ANNALISA DE GIROLAMO (Unità di personale interno)
• MICHELE SOLFRIZZO (Unità di personale interno)
• VERONICA MARIA TERESA LATTANZIO (Unità di personale interno)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of chromatography. B (Rivista)

Insieme di parole chiave di

• Keywords of "LC-MS/MS characterization of the urinary excretion profile of the mycotoxin deoxynivalenol in human and rat" (Insieme di parole chiave)