Amino-bonded silica as stationary phase for liquid chromatogrpahic determination of cyclopiazonic acid in fungal extracts (Articolo in rivista)

Type
Label
  • Amino-bonded silica as stationary phase for liquid chromatogrpahic determination of cyclopiazonic acid in fungal extracts (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Monaci L., Aresta A., Palmisano F., Visconti A., Zambonin C.G. (2002)
    Amino-bonded silica as stationary phase for liquid chromatogrpahic determination of cyclopiazonic acid in fungal extracts
    in Journal of chromatography (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Monaci L., Aresta A., Palmisano F., Visconti A., Zambonin C.G. (literal)
Pagina inizio
  • 79 (literal)
Pagina fine
  • 86 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 955 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Amino-bonded silica as stationary phase for liquid chromatogrpahic determination of cyclopiazonic acid in fungal extracts (literal)
Abstract
  • A new high-performance liquid (HPLC) chromatographic method is described as cyclopiazonic acid (CPA) determination in fungal cultures on a propylamino-bonded stationary phase with a CH3CN/CH3COONH4 buffer as mobile phase. Retention of CPA on propylamino modified silica under acidic conditions (protonated amino groups and deprotonated CPA) is governed by a mixed ion-exchange-reversed-phase mechanism. In addition to non-polar (hydrophobic) interactions, polar interactions with the surface silanols are also possible and become important as the polarity of the mobile phase decreases. A detection limit of 25 pg of CPA standard is obtained that represents an improvement of more than two orders of magnitude compared to existing HPLC procedures. UV-detector response was linear to 200 ng of CPA. Fungal extracts can be analysed after a simple dilution step with UV diode array detection that provides peak identity/purity assessment. The suitability of the proposed method as a rapid confirmatory test to assess the toxigenic potential of different Aspergillus and Penicillium strains is demonstrated by the analysis of 54 fungal extracts. (literal)
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