Nano-liquid chromatography in nutraceutical analysis: Determination of polyphenols in bee pollen (Articolo in rivista)

Type
Label
  • Nano-liquid chromatography in nutraceutical analysis: Determination of polyphenols in bee pollen (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.chroma. 2013.06.055 (literal)
Alternative label
  • C. Fanali, L. Dugo, A. Rocco (2013)
    Nano-liquid chromatography in nutraceutical analysis: Determination of polyphenols in bee pollen
    in Journal of chromatography (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • C. Fanali, L. Dugo, A. Rocco (literal)
Pagina inizio
  • 270 (literal)
Pagina fine
  • 274 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 1313 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 4 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Centro Integrato di Ricerca, Campus Bio-Medico University, Via Alvaro del Portillo 21, 00128 Rome, Italy b Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, 00015 Monterotondo, Italy (literal)
Titolo
  • Nano-liquid chromatography in nutraceutical analysis: Determination of polyphenols in bee pollen (literal)
Abstract
  • In this study, a nano-liquid chromatography based method for the simultaneous separation of 16 polyphenols employing UV-vis detection has been developed. A 100 ?m I.D. capillary column packed with C18 core-shell particles (2.6 ?m particle size, 100A? ) for 10 cm was employed. The separation of analytes was performed with a step gradient in less than 20 min, using 0.5% formic acid aqueous solution and acetonitrile as eluents. The optimized analytical method was validated and the resulting RSD% for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 4.68 and 5.57%, respectively. LOD and LOQ values were as low as 0.78 and 3.12 ?g/mL, while linearity, assessed in the concentration range of interest for all analytes, gave R2 >= 0.990. The method was finally applied to the analysis of polyphenols extracted from a collected bee pollen. Nine polyphenols, namely o-, p-coumaric acid, ferulic acid, myricetin, cinnamic acid, quercetin, naringenin, hesperitin and kaempferol, were identified. All analytes, with the exception of p-coumaric acid and myricetin, which partially co-eluted with other pollen components, were also quantified in the sample. (literal)
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