Identification of an Immunodominant IgE Epitope of the Parietaria judaica Major Allergen (Articolo in rivista)

Type
Label
  • Identification of an Immunodominant IgE Epitope of the Parietaria judaica Major Allergen (Articolo in rivista) (literal)
Anno
  • 1998-01-01T00:00:00+01:00 (literal)
Alternative label
  • Colombo P, Kennedy D, Ramsdale T, Costa MA, Duro G, Izzo V, Salvadori S, Guerrini R, Cocchiara R, Mirisola MG, Wood S, Geraci D (1998)
    Identification of an Immunodominant IgE Epitope of the Parietaria judaica Major Allergen
    in The Journal of immunology (1950)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Colombo P, Kennedy D, Ramsdale T, Costa MA, Duro G, Izzo V, Salvadori S, Guerrini R, Cocchiara R, Mirisola MG, Wood S, Geraci D (literal)
Pagina inizio
  • 2780 (literal)
Pagina fine
  • 2785 (literal)
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  • 160 (literal)
Rivista
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  • 6 (literal)
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  • 6 (literal)
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  • IBIM - CNR (literal)
Titolo
  • Identification of an Immunodominant IgE Epitope of the Parietaria judaica Major Allergen (literal)
Abstract
  • Par j 1.0101 is one of the two major allergens of the Parietaria judaica (Pj) pollen, and its three-dimensional structure was built by three-dimensional structural homology modeling. The resultant model was used to identify putative IgE binding regions. Western blot analysis of gene fragmentation products showed that the 1 to 30 region was capable of binding specific IgE from a pool of sera (n = 30) of patients allergic to Pj pollen. Using the structural model as a guide, deletion and site-directed mutagenesis of the 1 to 30 region was performed, and the amino acids involved in IgE binding were identified. In addition, a synthetic peptide covering the 1 to 30 region was capable of binding human IgE without triggering histamine release from basophils of Pj allergic patients (n = 6) and thus represents a haptenic molecule with potential use as an immunotolerant agent. This epitope is also present on the Par j 2.0101 major allergen representing a common IgE epitope. It is an immunodominant epitope, since it was capable of inhibiting 30% of all specific IgE against the Pj major allergens, and therefore, it might be a candidate for the future development of immunotherapeutics. (literal)
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