http://www.cnr.it/ontology/cnr/individuo/prodotto/ID200264

Detection of Grapevine leafroll-associated virus 7 using real time qRT-PCR and conventional RT-PCR. (Articolo in rivista)

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Detection of Grapevine leafroll-associated virus 7 using real time qRT-PCR and conventional RT-PCR. (Articolo in rivista) (literal)

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Alternative label

Al Rwahnih M., Osman F., Sudarshana M., Uyemoto J., Minafra A., Saldarelli P., Martelli G., Rowhani A. (2012)
Detection of Grapevine leafroll-associated virus 7 using real time qRT-PCR and conventional RT-PCR.
in Journal of virological methods; ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Al Rwahnih M., Osman F., Sudarshana M., Uyemoto J., Minafra A., Saldarelli P., Martelli G., Rowhani A. (literal)

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Note

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A.R.M., O.F. & R.A.: DEPARTMENT OF PLANT PATHOLOGY, UNIVERSITY OF CALIFORNIA, DAVIS, CALIFORNIA, USA. SM, UJ: USDA-ARS, UNIVERSITY OF CALIFORNIA, DAVIS, CA, USA M.A. &, S.P.: ISTITUTO DI VIROLOGIA VEGETALE, UOS DI BARI, CNR, ITALY M.G.: ISTITUTO DI VIROLOGIA VEGETALE, UOS DI BARI, CNR, ITALY & DIPARTIMENTO DI PROTEZIONE DELLE PIANTE E MICROBIOLOGIA APPLICATA, UNIVERSITA' DEGLI STUDI DI BARI, BARI, ITALY (literal)

Titolo

Detection of Grapevine leafroll-associated virus 7 using real time qRT-PCR and conventional RT-PCR. (literal)

Abstract

Nine isolates of Grapevine leafroll-associated virus 7 (GLRaV-7) from diverse geographical regions were sequenced to design more sensitive molecular diagnostic tools. The coat protein (CP) and heat shock protein 70 homologue (HSP70h) genes of these nine isolates were sequenced. Sequences were then used to design more sensitive molecular diagnostic tools. Sequence identity among these isolates ranged between 90 to 100% at the nucleotide and amino acid levels. One RT-PCR and two qRT-PCR assays were used to survey 86 different grapevines from the University of California, Davis Grapevine Virus Collection, the Foundation Plant Services collection and the USDA National Clonal Germplasm Repository, Davis, CA with primers designed in conserved regions of the CP and HSP70h genes. Results revealed that qRT-PCR assays designed in the HSP70h gene was more sensitive (29.07% positives) than that designed in the CP gene (22.09% positives) and both qRT-PCR assays proved to be more sensitive than RT-PCR. (literal)

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