Use of vancomycin silica stationary phase in packed capillary electrochromatography I. Enantiomer separation of basic compounds (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Use of vancomycin silica stationary phase in packed capillary electrochromatography I. Enantiomer separation of basic compounds (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/1522-2683(200102)22:3<535::AID-ELPS535>3.0.CO;2-8 (literal)

Alternative label

• DESIDERIO C, ATURKI Z, FANALI S (2001)
  Use of vancomycin silica stationary phase in packed capillary electrochromatography I. Enantiomer separation of basic compounds
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• DESIDERIO C, ATURKI Z, FANALI S (literal)

Pagina inizio

• 535 (literal)

Pagina fine

• 543 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 22 (literal)

Rivista

• Electrophoresis (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IMC-CNR; IMC-CNR;IMC-CNR; (literal)

Titolo

• Use of vancomycin silica stationary phase in packed capillary electrochromatography I. Enantiomer separation of basic compounds (literal)

Abstract

• Chiral separation of basic compounds was achieved by using 75 or 100 ?m ID fusedsilica capillaries packed with a vancomycin-modified diol silica stationary phase. The capillary was firstly packed for about 12 cm with a slurry mixture composed of diolsilica (3:1) then with the vancomycin modified diol-silica (3:1) (23 cm), and finally with diol-silica (3:1) for about 2 cm. Frits were prepared by a heating wire at the two ends of the capillary; the detector window was prepared at 8.5 cm from the end of the capillary where vancomycin was not present. The influence of the mobile phase composition (pH and concentration, organic modifier type and concentration) on the velocity of the electroosmotic flow, chiral resolution and enantioselectivity was studied. Good enantiomeric resolution was achieved for atenolol, oxprenolol, propranolol, and venlafaxine using a mobile phase composition of 100 mm ammonium acetate solution (pH 6)/water/acetonitrile (5:5:90 v/v/v) while for terbutaline a mixture of 5:15:80 v/v/v provided the best separations. The use of methanol instead of acetonitrile caused a general increase of enantiomer resolution of the studied compounds together with a reduction of efficiency and detector response. However, the combination of acetonitrile and methanol in the mobile phase (as, e.g., 10% methanol and 80% acetonitrile) allowed to improve the enantiomer resolution with satisfactory detector response. (literal)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)

Autore CNR

• CLAUDIA DESIDERIO (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• ZEINEB ATURKI (Unità di personale interno)

Incoming links:

Autore CNR di

• ZEINEB ATURKI (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• CLAUDIA DESIDERIO (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)