Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Alternative label

• Donatella RM Negri 1; Alessandra Rossi 1; Maria Blasi 2,6; Zuleika Michelini 3; Pasqualina Leone 3; Maria Vincenza Chiantore 1; Silvia Baroncelli 3; Gemma Perretta 4; Andrea Cimarelli 5; Mary E Klotman 6; Andrea Cara 3 (2012)
  Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines
  in Retrovirology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Donatella RM Negri 1; Alessandra Rossi 1; Maria Blasi 2,6; Zuleika Michelini 3; Pasqualina Leone 3; Maria Vincenza Chiantore 1; Silvia Baroncelli 3; Gemma Perretta 4; Andrea Cimarelli 5; Mary E Klotman 6; Andrea Cara 3 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 9 (literal)

Rivista

• Retrovirology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 69 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 Department of Infectious, Parasitic and Immune-mediated Diseases, Viale Regina Elena 299, Rome 00161, Italy. 2 Department of Cell Biology and Neurosciences, Viale Regina Elena 299, Rome 00161, Italy. 3 Separtment of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, Rome 00161, Italy. 4 Istituto di Biologia Cellulare e Neurobiologia-CNR, c/o ENEA-Casaccia, Roma 00123, Italy. 5 Department of Human Virology, Ecole Normale Supérieure de Lyon, Lyon, France. 6 Department of Medicine, Duke University Medical Center, Durham, NC27710, USA. (literal)

Titolo

• Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines (literal)

Abstract

• Background: Integrase defective lentiviral vectors (IDLV) represent a promising delivery system for immunization purposes. Human dendritic cells (DC) are the main cell types mediating the immune response and are readily transduced by IDLV, allowing effective triggering of in vitro expansion of antigen-specific primed CD8+ T cells. However, IDLV expression in transduced DC is at lower levels than those of the integrase (IN) competent counterpart, thus requiring further improvement of IDLV for future use in the clinic. Results: In this paper we show that the addition of simian immunodeficiency (SIV)-Vpx protein in the vector preparation greatly improves transduction of human and simian DC, but not of murine DC, thus increasing the ability of transduced DC to act as functional antigen presenting cells, in the absence of integrated vector sequences. Importantly, the presence of SIV-Vpx allows for using lower dose of input IDLV during in vitro transduction, thus further improving the IDLV safety profile. Conclusions: These results have significant implications for the development of IDLV-based vaccines. (literal)

Prodotto di

• Procedure e metodologie di utilizzo non umani nelle neuroscienze (SV.P16.009.001) (Modulo)

Autore CNR

• GEMMA PERRETTA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GEMMA PERRETTA (Unità di personale interno)

Prodotto

• Procedure e metodologie di utilizzo non umani nelle neuroscienze (SV.P16.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Retrovirology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines" (Insieme di parole chiave)