http://www.cnr.it/ontology/cnr/individuo/prodotto/ID185596

PCR-BASED IMMORTALIZATION AND SCREENING OF HIERARCHICAL POOLS OF CDNAS (Articolo in rivista)

Type

Label

PCR-BASED IMMORTALIZATION AND SCREENING OF HIERARCHICAL POOLS OF CDNAS (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

DESPOSITO, M; MAZZARELLA, R; PENGUE, G; JONES, C; DURSO, M; SCHLESSINGER, D (1994)
PCR-BASED IMMORTALIZATION AND SCREENING OF HIERARCHICAL POOLS OF CDNAS
in Nucleic acids research; Oxford University Press, Oxford (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

DESPOSITO, M; MAZZARELLA, R; PENGUE, G; JONES, C; DURSO, M; SCHLESSINGER, D (literal)

Pagina inizio

Pagina fine

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Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110 WASHINGTON UNIV,SCH MED,CTR MED GENET,ST LOUIS,MO 63110 CNR,INT INST GENET & BIOPHYS,I-80125 NAPLES,ITALY (literal)

Titolo

PCR-BASED IMMORTALIZATION AND SCREENING OF HIERARCHICAL POOLS OF CDNAS (literal)

Abstract

Starting from sequences of at least 60 bp, PCR-based screening has been developed to recover cDNAs from libraries without the necessity for hybridization or extensive DNA extraction steps. The method maintains the indefinite availability of even scarce cDNA libraries and provides an estimate of the relative abundance of the mRNA species. Isolation of a cDNA clone can be done in less than a week. cDNAs were isolated that were cognate for fragments of expressed sequences and for an exon predicted from genomic sequence. (literal)

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