Protein chips for screening of protease-protease inhibitor interactions (Abstract/Poster in atti di convegno)

Type
Label
  • Protein chips for screening of protease-protease inhibitor interactions (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Cimaglia F; D'Urso OF; Shevelev A; Logrieco A; Visconti A; Santino A; Poltronieri P (2006)
    Protein chips for screening of protease-protease inhibitor interactions
    in COST 853 Agricultural biomarkers for array technology, Protein chip working group and management committee meeting, Wageningen, 19-20.6.2006
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Cimaglia F; D'Urso OF; Shevelev A; Logrieco A; Visconti A; Santino A; Poltronieri P (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Presentazione orale delle Attività del working group IV: protein chips, del COST 853: Agricultural biomarkers for Array Technology. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.cost853.ch/agendaWageningen06.htm (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Università del Salento Corso di dottorato in Nanotecnologie, INN, Lecce (literal)
Titolo
  • Protein chips for screening of protease-protease inhibitor interactions (literal)
Abstract
  • Two Kunitz-type inhibitors (KPI) of class A, five of class B, and six of class C, in mixed or high pure fraction, in glycerol 40%, were spotted in NaHCO3 in triplicate on glass slides at increasing concentration 0.1, 0.2, 0.4 ?g/?l. KPIs binding to proteases, and in vitro inhibition was detected. Presence of full-length PI proteins and correlation with total protein concentration was checked by western blot with anti-KPI B10 polyclonal antibodies. Physical interaction with trypsin and chymotrypsin was detected, differentially characterising three KPIs. Inhibition assays show PI protease activity at each concentration. On the chip, protein-protein interaction data provide additional information and screening of PIs: chips were useful to show that cathepsin B, a cysteine protease, was actively bound by PIs, information that allowed to test the in vitro inhibition. Kunitz-PIs may be evaluated on the chip with anti-KPI B10 antibodies, for homogeneous presentation of PI proteins and estimation of amount of protein bound to the surface. (literal)
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