Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloGeneration of a Functional Human Neural Network by NDM29 Overexpression in Neuroblastoma Cancer Cells
Anno di pubblicazione2017
Formato-
Autore/iAlloisio S, Garbati P, Viti F, Dante S, Barbieri R, Arnaldi G, Petrelli A, Gigoni A, Giannoni P, Quarto R, Nobile M, Vassalli M, Pagano A
Affiliazioni autoriETT Spa, via Sestri 37, 16154, Genoa, Italy National Research Council, Institute of Biophysics, via De Marini 6, 16149, Genoa, Italy IRCCS-AOU San Martino-IST, Genova, Italy Istituto Italiano di Tecnologia, Via Morego 30, I-16163, Genova, Italy Department of Experimental Medicine (DIMES), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy IRCCS-AOU San Martino-IST, Genova, Italy. aldo.pagano@unige.it Department of Experimental Medicine (DIMES), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy
Autori CNR e affiliazioni
  • SUSANNA ALLOISIO
  • MARIO NOBILE
  • MASSIMO VASSALLI
  • FEDERICA VITI
  • RAFFAELLA BARBIERI
Lingua/e
  • inglese
AbstractRecent advances in life sciences suggest that human and rodent cell responses to stimuli might differ significantly. In this context, the results achieved in neurotoxicology and biomedical research practices using neural networks obtained from mouse or rat primary culture of neurons would benefit of the parallel evaluation of the same parameters using fully differentiated neurons with a human genetic background, thus emphasizing the current need of neuronal cells with human origin. In this work, we developed a human functionally active neural network derived by human neuroblastoma cancer cells genetically engineered to overexpress NDM29, a non-coding RNAwhose increased synthesis causes the differentiation toward a neuronal phenotype. These cells are here analyzed accurately showing functional and morphological traits of neurons such as the expression of neuron-specific proteins and the possibility to generate the expected neuronal current traces and action potentials. Their morphometrical analysis is carried out by quantitative phase microscopy showing soma and axon sizes compatible with those of functional neurons. The ability of these cells to connect autonomously forming physical junctions recapitulates that of hippocampal neurons, as resulting by connect-ability test. Lastly, these cells self-organize in neural networks able to produce spontaneous firing, in which spikes can be clustered in bursts. Altogether, these results show that the neural network obtained by NDM29-dependent differentiation of neuroblastoma cells is a suitable tool for biomedical research practices.
Lingua abstractinglese
Altro abstract-
Lingua altro abstract-
Pagine da6097
Pagine a6106
Pagine totali10
RivistaMolecular neurobiology
Attiva dal 1987
Editore: Humana Press, - Clifton, NJ
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0893-7648
Titolo chiave: Molecular neurobiology
Titolo proprio: Molecular neurobiology.
Titolo abbreviato: Mol. neurobiol.
Numero volume della rivista54
Fascicolo della rivista8
DOI10.1007/s12035-016-0161-3
Verificato da referee-
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000409039000029)
Parole chiaveNeuroblastoma, Human neuronal network, Multi-electrode array (MEA), NDM29, SKNBE2-S1.1, Cancer
Link (URL, URI)https://link.springer.com/article/10.1007%2Fs12035-016-0161-3
Titolo parallelo-
Licenza-
Scadenza embargo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IBF — Istituto di biofisica
Moduli/Attività/Sottoprogetti CNR
  • DFM.AD004.021.001 : Processi di membrana nella comunicazione intra- ed inter- cellulare
Progetti Europei-
Allegati
Generation of a Functional Human Neural Network by NDM29 Overexpression in Neuroblastoma Cancer Cells (documento privato )
Tipo documento: application/pdf