Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloSerum IgE Reactivity Profiling in an Asthma Affected Cohort
Anno di pubblicazione2011
FormatoCartaceo
Autore/iDottorini, Tania; Sole, Gabriella; Nunziangeli, Luisa; Baldracchini, Francesca; Senin, Nicola; Mazzoleni, Giorgio; Proietti, Carla; Balaci, Lenuta; Crisanti, Andrea
Affiliazioni autori1 Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy, 2 National Research Council of Italy (CNR), Institute of Genetics and Biomedical Research (IRGB), Cagliari, Italy, 3 Microtest Matrices LtD, London, United Kingdom, 4 Department of Industrial Engineering, University of Perugia, Perugia, Italy, 5 Biological Sciences, Imperial College London, London, United Kingdom
Autori CNR e affiliazioni
  • LENUTA BALACI
  • GABRIELLA SOLE
Lingua/e
  • inglese
AbstractBackground: Epidemiological evidence indicates that atopic asthma correlates with high serum IgE levels though the contribution of allergen specific IgE to the pathogenesis and the severity of the disease is still unclear. Methods: We developed a microarray immunoassay containing 103 allergens to study the IgE reactivity profiles of 485 asthmatic and 342 non-asthmatic individuals belonging to families whose members have a documented history of asthma and atopy. We employed k-means clustering, to investigate whether a particular IgE reactivity profile correlated with asthma and other atopic conditions such as rhinitis, conjunctivitis and eczema. Methods: We developed a microarray immunoassay containing 103 allergens to study the IgE reactivity profiles of 485 asthmatic and 342 non-asthmatic individuals belonging to families whose members have a documented history of asthma and atopy. We employed k-means clustering, to investigate whether a particular IgE reactivity profile correlated with asthma and other atopic conditions such as rhinitis, conjunctivitis and eczema. Results: Both case-control and parent-to-siblings analyses demonstrated that while the presence of specific IgE against individual allergens correlated poorly with pathological conditions, particular reactivity profiles were significantly associated with asthma (p,10E-09). An artificial neural network (ANN)-based algorithm, calibrated with the profile reactivity data, correctly classified as asthmatic or non-asthmatic 78% of the individual examined. Multivariate statistical analysis demonstrated that the familiar relationships of the study population did not affect the observed correlations. Conclusions: These findings indicate that asthma is a higher-order phenomenon related to patterns of IgE reactivity rather than to single antibody reactions. This notion sheds new light on the pathogenesis of the disease and can be readily employed to distinguish asthmatic and non-asthmatic individuals on the basis of their serum reactivity profile.
Lingua abstractinglese
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Pagine da-
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Pagine totali11
RivistaPloS one
Attiva dal 2006
Editore: Public Library of Science - San Francisco, CA
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1932-6203
Titolo chiave: PloS one
Titolo proprio: PloS one
Titolo abbreviato: PLoS ONE
Titoli alternativi:
  • Public Library of Science one
  • PLoS 1
Numero volume della rivista6
Fascicolo della rivista8
DOI10.1371/journal.pone.0022319
Verificato da refereeSì: Internazionale
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000293561200013)
Parole chiaveasthma, IgE reactivity, microarray immunoassay
Link (URL, URI)-
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Strutture CNR
  • IRGB — Istituto di Ricerca Genetica e Biomedica
Moduli/Attività/Sottoprogetti CNR
  • ME.P07.006.002 : Utilizzo della popolazione sarda, per la sua omogeneità, per lo studio delle basi genetiche dell'asma allergico
Progetti Europei-
Allegati