Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloRapid diagnosis of primary ciliary dyskinesia: cell culture and soft computing analysis
Anno di pubblicazione2013
Formato
  • Elettronico
  • Cartaceo
Autore/iPifferi M, Bush A, Montemurro F, Pioggia G, Piras M, Tartarisco G, Cicco MD, Chinellato I, Cangiotti AM, Boner AL
Affiliazioni autoriConsiglio Nazionale delle Ricerche (CNR)
Autori CNR e affiliazioni
  • GIOVANNI PIOGGIA
  • GENNARO TARTARISCO
Lingua/e
  • inglese
AbstractDiagnosis of primary ciliary dyskinesia (PCD) sometimes requires repeated nasal brushing to exclude secondary ciliary alterations. Our aim was to evaluate whether the use of a new method of nasal epithelial cell culture can speed PCD diagnosis in doubtful cases and to identify which are the most informative parameters by means of a multilayer artificial neural network (ANN). A cross-sectional study was performed in patients with suspected PCD. All patients underwent nasal brushing for ciliary motion analysis, ultrastructural assessment and evaluation of ciliary function after ciliogenesis in culture by ANN. 151 subjects were studied. A diagnostic suspension cell culture was obtained in 117 nasal brushings. A diagnosis of PCD was made in 36 subjects (29 of whom were children). In nine out of the 36 patients the diagnosis was made only after a second brushing, because of equivocal results of both tests at first examination. In each of these subjects diagnosis of PCD was confirmed by cell culture results. Cell culture in suspension evaluated by means of ANN allows the separation of PCD from secondary ciliary dyskinesia patients after only 5 days of culture and allows diagnosis to be reached in doubtful cases, thus avoiding the necessity of a second sample.
Lingua abstractinglese
Altro abstract-
Lingua altro abstract-
Pagine da960
Pagine a965
Pagine totali-
RivistaThe European respiratory journal
Attiva dal 1988
Editore: European Respiratory Society Journals Ltd.. - Lausanne
Lingua: inglese
ISSN: 0903-1936
Titolo chiave: The European respiratory journal
Titolo proprio: The European respiratory journal.
Titolo abbreviato: Eur. respir. j.
Numero volume della rivista41
Fascicolo della rivista4
DOI10.1183/09031936.00039412
Verificato da refereeSì: Internazionale
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • PubMed (Codice:22903970)
  • ISI Web of Science (WOS) (Codice:000317639000028)
Parole chiaveCiliary motion analysis; mathematical evaluation; suspension cell culture; transmission electron microscopy
Link (URL, URI)-
Titolo parallelo-
Licenza-
Scadenza embargo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • ISASI — Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello"
  • IFC — Istituto di fisiologia clinica
Moduli/Attività/Sottoprogetti CNR
  • ME.P01.015.002 : Pervasive healthcare and biomedical engineering
Progetti Europei-
Allegati
Rapid diagnosis of primary ciliary dyskinesia: cell culture and soft computing analysis (documento privato )
Tipo documento: application/pdf

Dati associati a vecchie tipologie
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Editore
  • ERS, European respiratory society, Sheffield (Regno Unito)