Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloAtomic force microscopy for biomechanical and structural analysis of human dermis: A complementary tool for medical diagnosis and therapy monitoring
Anno di pubblicazione2018
Autore/iPenuela, Leonardo; Negro, Carola; Massa, Michela; Repaci, Erica; Cozzani, Emanuele; Parodi, Aurora; Scaglione, Silvia; Quarto, Rodolfo; Raiteri, Roberto
Affiliazioni autoriUniv Genoa; Univ Genoa; Univ Genoa; IEIIT Inst CNR IEIIT Genoa
Autori CNR e affiliazioni
  • inglese
AbstractSkin mechanical properties are usually measured considering the entire skin thickness and very little is known about the mechanical behaviour of individual skin layers. We propose atomic force microscopy (AFM) as a tool to quantify nanoscale changes in the biomechanical properties and ultrastructure of human papillary dermis exposed to different mechanical and physical stimuli. Samples from 3 human skin biopsies were studied: one stretched by obesity, one subjected to a high level of sun exposure and normal skin as control. Slices of the papillary dermis layer were harvested at controlled depths from each skin biopsy and 25m(2) areas of each slice were imaged and D-periodicity of collagen fibres measured by AFM, together with their stiffness. Standard histological analysis was also carried out to correlate biochemical properties and their distribution with stiffness and topography. We obtained similar stiffness values between the sample affected by obesity and the control sample at any depth level into the dermis, while the sun-exposed sample presented a significantly lower stiffness. Additionally, all samples presented an increase in the stiffness at higher depths into the papillary dermis layer. Collagen fibres close to the epidermis of sample affected either by obesity and sun exposurethe former even more than the latterare thicker and present a larger D-period than those in the control sample. Our results open the possibility to use structural and mechanical analysis based on AFM as a complementary tool for medical diagnosis and therapy monitoring.
Lingua abstractinglese
Altro abstract-
Lingua altro abstract-
Pagine da150
Pagine a155
Pagine totali6
RivistaExperimental dermatology
Attiva dal 1992
Editore: Munksgaard, - Oxford
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0906-6705
Titolo chiave: Experimental dermatology
Titolo proprio: Experimental dermatology.
Titolo abbreviato: Exp. dermatol.
Numero volume della rivista27
Fascicolo della rivista2
Verificato da refereeSì: Internazionale
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000423679700006)
Parole chiavebiomechanics, dermis, nano-indentation, stiffness, ultrastructure
Link (URL, URI)-
Titolo parallelo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
Moduli CNR
    Progetti Europei-