Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloA new cell-laden 3D Alginate-Matrigel hydrogel resembles human breast cancer cell malignant morphology, spread and invasion capability observed "in vivo"
Anno di pubblicazione2018
FormatoElettronico
Autore/iCavo, Marta; Caria, Marco; Pulsoni, Ilaria; Beltrame, Francesco; Fato, Marco; Scaglione, Silvia
Affiliazioni autoriNatl Res Council CNR; Univ Genoa; React4life Srl
Autori CNR e affiliazioni
  • MARCO MASSIMO FATO
  • FRANCESCO BELTRAME
  • MARTA MARIA CAVO
  • SILVIA SCAGLIONE
Lingua/e
  • inglese
AbstractPurpose of this study was the development of a 3D material to be used as substrate for breast cancer cell culture. We developed composite gels constituted by different concentrations of Alginate (A) and Matrigel (M) to obtain a structurally stable-in-time and biologically active substrate. Human aggressive breast cancer cells (i.e. MDA-MB-231) were cultured within the gels. Known the link between cell morphology and malignancy, cells were morphologically characterized and their invasiveness correlated through an innovative bioreactor-based invasion assay. A particular type of gel (i.e. 50% Alginate, 50% Matrigel) emerged thanks to a series of significant results: 1. cells exhibited peculiar cytoskeleton shapes and nuclear fragmentation characteristic of their malignancy; 2. cells expressed the formation of the so-called invadopodia, actin-based protrusion of the plasma membrane through which cells anchor to the extracellular matrix; 3. cells were able to migrate through the gels and attach to an engineered membrane mimicking the vascular walls hosted within bioreactor, providing a completely new 3D in vitro model of the very precursor steps of metastasis.
Lingua abstractinglese
Altro abstract-
Lingua altro abstract-
Pagine da-
Pagine a-
Pagine totali12
RivistaScientific reports (Nature Publishing Group)
Attiva dal 2011
Editore: Nature Publishing Group - London
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 2045-2322
Titolo chiave: Scientific reports (Nature Publishing Group)
Titolo proprio: Scientific reports (Nature Publishing Group)
Numero volume della rivista8
Fascicolo della rivista-
DOI10.1038/s41598-018-23250-4
Verificato da refereeSì: Internazionale
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000428518900030)
Parole chiavetissue engineering; hydrogels; cancer
Link (URL, URI)-
Titolo parallelo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
Moduli CNR
    Progetti Europei-
    Allegati