Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloDesign and characterization of a tissue-engineered bilayer scaffold for osteochondral tissue repair
Anno di pubblicazione2015
FormatoElettronico
Autore/iGiannoni, Paolo; Lazzarini, Erica; Lazzarini, Erica; Ceseracciu, Luca; Barone, Alberto C.; Quarto, Rodolfo; Quarto, Rodolfo; Scaglione, Silvia; Scaglione, Silvia
Affiliazioni autoriCentro di Biotecnologie Avanzate; Universita degli Studi di Genova; Istituto Italiano di Tecnologia; Istituto Di Scienza E Tecnologia Dei Materiali Ceramici, Faenza; Consiglio Nazionale delle Ricerche
Autori CNR e affiliazioni
  • SILVIA SCAGLIONE
Lingua/e
  • inglese
AbstractTreatment of full-thickness cartilage defects relies on osteochondral bilayer grafts, which mimic the microenvironment and structure of the two affected tissues: articular cartilage and subchondral bone. However, the integrity and stability of the grafts are hampered by the presence of a weak interphase, generated by the layering processes of scaffold manufacturing. We describe here the design and development of a bilayer monolithic osteochondral graft, avoiding delamination of the two distinct layers but preserving the cues for selective generation of cartilage and bone. A highly porous polycaprolactone-based graft was obtained by combining solvent casting/particulate leaching techniques. Pore structure and interconnections were designed to favour in vivo vascularization only at the bony layer. Hydroxyapatite granules were added as bioactive signals at the site of bone regeneration. Unconfined compressive tests displayed optimal elastic properties and low residual deformation of the graft after unloading (< 3%). The structural integrity of the graft was successfully validated by tension fracture tests, revealing high resistance to delamination, since fractures were never displayed at the interface of the layers (n=8). Ectopic implantation of grafts in nude mice, after seeding with bovine trabecular bone-derived mesenchymal stem cells and bovine articular chondrocytes, resulted in thick areas of mature bone surrounding ceramic granules within the bony layer, and a cartilaginous alcianophilic matrix in the chondral layer. Vascularization was mostly observed in the bony layer, with a statistically significant higher blood vessel density and mean area. Thus, the easily generated osteochondral scaffolds, since they are mechanically and biologically functional, are suitable for tissue-engineering applications for cartilage repair.
Lingua abstractinglese
Altro abstract-
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Pagine da1182
Pagine a1192
Pagine totali-
RivistaJournal of tissue engineering and regenerative medicine
Attiva dal 2007
Editore: John Wiley & Sons - Chichester, West Sussex, UK
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1932-6254
Titolo chiave: Journal of tissue engineering and regenerative medicine
Titolo proprio: Journal of tissue engineering and regenerative medicine.
Titolo abbreviato: J. tissue eng. regen. med.
Numero volume della rivista9
Fascicolo della rivista10
DOI10.1002/term.1651
Verificato da refereeSì: Internazionale
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • Scopus (Codice:2-s2.0-84944514656)
Parole chiaveBone tissue engineering, Cartilage tissue engineering, Interface, Osteochondral, Porosity, Progenitor cell, Scaffold
Link (URL, URI)http://www.scopus.com/record/display.url?eid=2-s2.0-84944514656&origin=inward
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Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
Moduli CNR-
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