Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloEfficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering.
Anno di pubblicazione2017
Autore/iRothrauff, Benjamin B; Coluccino, Luca; Gottardi, Riccardo; Ceseracciu, Luca; Scaglione, Silvia; Goldoni, Luca; Tuan, Rocky S
Affiliazioni autoriCenter for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Autori CNR e affiliazioni
  • inglese
AbstractTissue engineering using adult mesenchymal stem cells (MSCs), a promising approach for cartilage repair, is highly dependent on the nature of the matrix scaffold. Thermoresponsive, photocrosslinkable hydrogels were fabricated by functionalizing pepsin-soluble decellularized tendon and cartilage extracellular matrices (ECM) with methacrylate groups. Methacrylated gelatin hydrogels served as controls. When seeded with human bone marrow MSCs and cultured in chondrogenic medium, methacrylated ECM hydrogels experienced less cell-mediated contraction, as compared against non-methacrylated ECM hydrogels. However, methacrylation slowed or diminished chondrogenic differentiation of seeded MSCs, as determined through analyses of gene expression, biochemical composition and histology. In particular, methacrylated cartilage hydrogels supported minimal due to chondrogenesis over 42 weeks, as hydrogel disintegration beginning at day 14 presumably compromised cell-matrix interactions. As compared against methacrylated gelatin hydrogels, MSCs cultured in non-methacrylated ECM hydrogels exhibited comparable expression of chondrogenic genes (Sox9, Aggrecan and collagen type II) but increased collagen type I expression. Non-methacrylated cartilage hydrogels did not promote chondrogenesis to a greater extent than either non-methacrylated or methacrylated tendon hydrogels. Whereas methacrylated gelatin hydrogels supported relatively homogeneous increases in proteoglycan and collagen type II deposition throughout the construct over 42 days, ECM hydrogels possessed greater heterogeneity of staining intensity and construct morphology. These results do not support the utility of pepsin-solubilized cartilage and tendon hydrogels for cartilage tissue engineering over methacrylated gelatin hydrogels. Methacrylation of tendon and cartilage ECM hydrogels permits thermal- and light-induced polymerization but compromises chondrogenic differentiation of seeded MSCs.
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RivistaJournal of tissue engineering and regenerative medicine (Online)
Attiva dal 2007
Editore: John Wiley & Sons, Ltd., - [Chichester, West Sussex UK]
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1932-7005
Titolo chiave: Journal of tissue engineering and regenerative medicine (Online)
Titolo proprio: Journal of tissue engineering and regenerative medicine (Online)
Titolo abbreviato: J. tissue eng. regen. med. (Online)
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Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:28486778)
Parole chiavetissue engineering
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Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
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