Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloDesign of Decorated Self-Assembling Peptide Hydrogels as Architecture for Mesenchymal Stem Cells
Anno di pubblicazione2016
Formato-
Autore/iZamuner, Annj; Cavo, Marta; Scaglione, Silvia; Messina, Grazia Maria Lucia; Russo, Teresa; Gloria, Antonio; Marletta, Giovanni; Dettin, Monica
Affiliazioni autoriUniv Padua; Natl Res Council CNR; Univ Genoa; Univ Catania; CSGI; CNR
Autori CNR e affiliazioni
  • MARTA MARIA CAVO
  • ANTONIO GLORIA
  • SILVIA SCAGLIONE
  • TERESA RUSSO
Lingua/e
  • inglese
AbstractHydrogels from self-assembling ionic complementary peptides have been receiving a lot of interest from the scientific community as mimetic of the extracellular matrix that can offer three-dimensional supports for cell growth or can become vehicles for the delivery of stem cells, drugs or bioactive proteins. In order to develop a 3D "architecture" for mesenchymal stem cells, we propose the introduction in the hydrogel of conjugates obtained by chemoselective ligation between a ionic-complementary self-assembling peptide (called EAK) and three different bioactive molecules: an adhesive sequence with 4 Glycine-Arginine-Glycine-Aspartic Acid-Serine-Proline (GRGDSP) motifs per chain, an adhesive peptide mapped on h-Vitronectin and the growth factor Insulin-like Growth Factor-1 (IGF-1). The mesenchymal stem cell adhesion assays showed a significant increase in adhesion and proliferation for the hydrogels decorated with each of the synthesized conjugates; moreover, such functionalized 3D hydrogels support cell spreading and elongation, validating the use of this class of self-assembly peptides-based material as very promising 3D model scaffolds for cell cultures, at variance of the less realistic 2D ones. Furthermore, small amplitude oscillatory shear tests showed that the presence of IGF-1-conjugate did not alter significantly the viscoelastic properties of the hydrogels even though differences were observed in the nanoscale structure of the scaffolds obtained by changing their composition, ranging from long, well-defined fibers for conjugates with adhesion sequences to the compact and dense film for the IGF-1-conjugate.
Lingua abstractinglese
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Pagine da-
Pagine a-
Pagine totali18
RivistaMaterials (Basel)
Attiva dal 2008
Editore: Molecular Diversity Preservation International - Basel
Lingua: inglese
ISSN: 1996-1944
Titolo chiave: Materials (Basel)
Titolo proprio: Materials. (Basel)
Titolo abbreviato: Materials (Basel)
Numero volume della rivista9
Fascicolo della rivista9
DOI10.3390/ma9090727
Verificato da referee-
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000382262000017)
Parole chiaveself-assembling peptides, mesenchymal stem cells, bio-transamination, chemoselective ligation, IGF-1, vitronectin, RGD
Link (URL, URI)-
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Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
Moduli CNR-
Progetti Europei-
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