Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloBioactive TGF-beta1/ hydroxyapatite alginate-based scaffolds for osteochondral tissue repair: design, realization and multi-level characterization
Anno di pubblicazione2016
Autore/iColuccino L, Stagnaro P, Vassalli M, Scaglione S
Affiliazioni autoriCNR IEIIT; CNR IBF; CNR ISMAC
Autori CNR e affiliazioni
  • inglese
AbstractBackground: The design of an appropriate microenvironment for stem cell differentiation constitutes a multitask mission and a critical step toward the clinical application of tissue substitutes. With the aim of producing a bioactive material for orthopedic applications, a transforming growth factor-? (TGF- ?1)/hydroxyapatite (HA) association within an alginate-based scaffold was investigated. The bioactive scaffold was carefully designed to offer specific biochemical cues for an efficient and selective cell differentiation toward the bony and chondral lineages. Methods: Highly porous alginate scaffolds were fabricated from a mixture of calcium cross-linked alginates by means of a freeze-drying technique. In the chondral layer, the TGF in citric acid was mixed with an alginate/ alginate-sulfate solution. In the bony layer, HA granules were added as bioactive signal, to offer an osteoinductive surface to the cells. Optical and scanning electron microscopy analyses were performed to assess the macromicro architecture of the biphasic scaffold. Different mechanical tests were conducted to evaluate the elastic modulus of the grafts. For the biological validation of the developed prototype, mesenchymal stem cells were loaded onto the samples; cellular adhesion, proliferation and in vivo biocompatibility were evaluated. Results and conclusions: The results successfully demonstrated the efficacy of the designed osteochondral graft, which combined interesting functional properties and biomechanical performances, thus becoming a promising candidate for osteochondral tissue-engineering applications.
Lingua abstractinglese
Altro abstract-
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Pagine daE42
Pagine aE52
Pagine totali-
RivistaJ Appl Biomater Function Mater
Attiva dal 2012
Editore: Wichtig Editore
Paese di pubblicazione: Italia
Lingua: inglese
ISSN: 2280-8000
Titolo chiave: J Appl Biomater Function Mater
Numero volume della rivista14
Fascicolo della rivista1
Verificato da refereeSì: Internazionale
Stato della pubblicazionePublished version
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000378077900006)
Parole chiavetissue engineering; materials; bioeingineering
Link (URL, URI)
Titolo parallelo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IBF — Istituto di biofisica
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
  • ISMAC — ISMAC - Sede secondaria di Genova
Moduli CNR
    Progetti Europei-