Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloDissociated effects of glucose-dependent insulinotropic polypeptide vs glucagon-like peptide-1 on beta-cell secretion and insulin clearance in mice
Anno di pubblicazione2010
Formato-
Autore/iPacini, G.; Thomaseth, K.; Ahren, B.
Affiliazioni autori1, 2: ISIB-CNR
Autori CNR e affiliazioni
  • GIOVANNI PACINI
  • KARL THOMASETH
Lingua/e-
AbstractGlucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) potently augment insulin response to glucose. It is less known what their effects are insulin clearance, which also contributes to peripheral hyperinsulinemia observed after administration of incretins together with glucose. The aims of this study were the quantification of C-peptide secretion and the evaluation of insulin clearance after administration of GIP with glucose. This allows the assessment of GIP's effects on hyperinsulinemia. In addition, GIP's effects were compared with those of GLP-1. Anesthetized female NMRI mice were injected intravenously with glucose alone (1 g/kg, n = 35) or glucose together with GIP (50 mug/kg, n = 12). Samples were taken through the following 50 minutes, and C-peptide and insulin concentrations were used to reconstruct C-peptide secretion rate and insulin clearance. In a previous study, GLP-1 (10 mug/kg) was used in 12 mice; and we used those GLP-1 results to compare GIP effects with those of GLP-1. C-peptide secretion rate peaked at 1 minute after glucose injection, and the immediate part of the insulin-releasing process was markedly augmented by both incretin hormones (1-minute suprabasal increment secretory rate was 20 +/- 2 pmol/min for GIP and 28 +/- 2 pmol/min for GLP-1, vs only 9 +/- 1 pmol/min for glucose alone; P < .001). Until 10 minutes after administration, C-peptide secretion remained higher with incretins (P < .0001), whereas starting from 20 minutes, the 3 patterns were undistinguishable (P > .2). Insulin clearance, previously shown to be abridged by 46% with GLP-1, was reduced only by a nonsignificant (P = .27) 21% with GIP. This study thus shows that the 2 incretins markedly augment glucose-stimulated insulin secretion in mice by a preferential action on the immediate response to glucose of insulin secretion. However, the action of GIP is less effective than that of GLP-1. Insulin clearance with GIP is not significantly reduced. We conclude that GIP is less potent than GLP-1 in inducing glucose-stimulated hyperinsulinemia in the mouse.
Lingua abstract-
Altro abstract-
Lingua altro abstract-
Pagine da988
Pagine a92
Pagine totali-
RivistaMetabolism, clinical and experimental (Online)
Attiva dal 1952
Editore: W.B. Saunders - [Philadelphia, Pa.]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1532-8600
Titolo chiave: Metabolism, clinical and experimental (Online)
Titolo proprio: Metabolism, clinical and experimental (Online)
Titolo abbreviato: Metab. clin. exper. (Online)
Titolo alternativo: Metabolism (Online)
Numero volume della rivista59
Fascicolo della rivista-
DOI10.1016/j.metabol.2009.10.021
Verificato da referee-
Stato della pubblicazione-
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000278854400009)
Parole chiave-
Link (URL, URI)-
Titolo parallelo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
  • ISIB — Istituto di ingegneria biomedica
Moduli CNR
    Progetti Europei-
    Allegati

      Dati storici
      I dati storici non sono modificabili, sono stati ereditati da altri sistemi (es. Gestione Istituti, PUMA, ...) e hanno solo valore storico.
      Rivista ISIMETABOLISM [12789J0]