Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloOriented collagen nanocoatings for tissue engineering
Anno di pubblicazione2014
Autore/iPastorino, Laura; Dellacasa, Elena; Scaglione, Silvia; Giulianelli, Massimo; Sbrana, Francesca; Vassalli, Massimo; Ruggiero, Carmelina
Affiliazioni autoriUniversity of Genoa; Consiglio Nazionale delle Ricerche (CNR); Consiglio Nazionale delle Ricerche (CNR)
Autori CNR e affiliazioni
AbstractCollagens are among the most widely present and important proteins composing the human total body, providing strength and structural stability to various tissues, from skin to bone. In this paper, we report an innovative approach to bioactivate planar surfaces with oriented collagen molecules to promote cells proliferation and alignment. The Langmuir-Blodgett technique was used to form a stable collagen film at the air-water interface and the Langmuir-Schaefer deposition was adopted to transfer it to the support surface. The deposition process was monitored by estimating the mass of the protein layers after each deposition step. Collagen films were then structurally characterized by atomic force, scanning electron and fluorescent microscopies. Finally, collagen films were functionally tested in vitro. To this aim, 3T3 cells were seeded onto the silicon supports either modified or not (control) by collagen film deposition. Cells adhesion and proliferation on collagen films were found to be greater than those on control both after 1 (p < 0.05) and 7 days culture. Moreover, the functionalization of the substrate surface triggered a parallel orientation of cells when cultured on it. In conclusion, these data demonstrated that the Langmuir-Schaefer technique can be successfully used for the deposition of oriented collagen films for tissue engineering applications. (C) 2013 Elsevier B.V. All rights reserved.
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Pagine da372
Pagine a378
Pagine totali7
RivistaColloids and surfaces. B, Biointerfaces (Print)
Attiva dal 1993
Editore: Elsevier - Tokyo
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0927-7765
Titolo chiave: Colloids and surfaces. B, Biointerfaces (Print)
Titolo proprio: Colloids and surfaces. (Print)
Titolo abbreviato: Colloids surf., B Biointerfaces (Print)
Numero volume della rivista114
Fascicolo della rivista-
Verificato da referee-
Stato della pubblicazione-
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000331596100049)
Parole chiaveTissue engineering, Collagen, Langmuir Blodgett technique, Cellular response
Link (URL, URI)
Titolo parallelo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IBF — Istituto di biofisica
  • IEIIT — IEIIT - Sede secondaria di Genova
Moduli/Attività/Sottoprogetti CNR
  • MD.P01.001.001 : Processi di membrana nella comunicazione intra- ed inter-cellulare
  • INT.P02.002.004 : Approcci innovativi al tissue engineering
Progetti Europei-
Oriented collagen nanocoatings for tissue engineering (documento privato )
Descrizione: articolo originale
Tipo documento: application/pdf