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Tipo di prodottoArticolo in rivista
TitoloCharacterization of the in vitro propagation of epileptiform electrophysiological activity in organotypic hippocampal slice cultures coupled to 3D-microelectrode arrays
Anno di pubblicazione2010
Autore/iPisciotta Marzia; Morgavi Giovanna; Jahnsen Herik
Affiliazioni autoriPisciotta Marzia, Herik Jahnsen Division of Neurophysiology, Department of Neuroscience and Pharmacology, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark
Autori CNR e affiliazioni
  • inglese
AbstractDynamic aspects of the propagation of epileptiform activity have so far received little attention. With the aim of providing new insights about the spatial features of the propagation of epileptic seizures in the nervous system, we studied in vitro the initiation and propagation of traveling epileptiform waves of electrophysiological activity in the hippocampus by means of substrate three-dimensional microelectrode arrays (MEAs) for extracellular measurements. Pharmacologically disinhibited hippocampal slices spontaneously generate epileptiform bursts mostly originating in CA3 and propagating to CA1 Our study specifically addressed the activity-dependent changes of the propagation of traveling electrophysiological waves in organotypic hippocampal slices during epileptiform discharge and in particular our question is: what happens to the epileptic signals during their propagation through the slice? Multichannel data analysis enabled us to quantify an activity-dependent increase in the propagation velocity of spontaneous bursts. Moreover, through the evaluation of the coherence of the signals, it was possible to point out that only the lower-frequency components (<95 Hz) of the electrical activity are completely coherent with respect to the activity originating in the CA3, while components at higher frequencies lose the coherence, possibly suggesting that the cellular mechanism mediating propagation of electrophysiological activity becomes ineffective for those firing rates exceeding an upper bound or that some noise of neuronal origin was added to the signal during propagation. (C) 2010 Elsevier B.V. All rights reserved.
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Pagine da46
Pagine a54
Pagine totali-
RivistaBrain research
Attiva dal 1966
Editore: Elsevier - Shannon ;
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0006-8993
Titolo chiave: Brain research
Titolo proprio: Brain research.
Titolo abbreviato: Brain res.
Numero volume della rivista1358
Fascicolo della rivista-
Verificato da refereeSì: Internazionale
Stato della pubblicazione-
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000283814800006)
  • PubMed (Codice:20713026)
  • Scopus (Codice:2.0-77957352774)
Parole chiaveHippocampus, Epileptiform activity, Microelectrode arrays, Propagation velocity, Coherence analysis
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Strutture CNR
  • IEIIT — IEIIT - Sede secondaria di Genova
Moduli CNR
    Progetti Europei-
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    Dati storici
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    Area disciplinareComputer Science & Engineering
    Area valutazione CIVRIngegneria industriale e informatica
    Rivista ISIBRAIN RESEARCH [21362J0]