Consiglio Nazionale delle Ricerche

Tipo di prodottoArticolo in rivista
TitoloAlternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping
Anno di pubblicazione2003
FormatoCartaceo
Autore/iP. Ferro, A. Forlani, M. Muselli, U. Pfeffer
Affiliazioni autoriP. Ferro: Laboratory of Molecular Oncology, National Cancer Research Institute, I-16132 Genova, Italy; A. Forlani: Laboratory of Molecular Oncology, National Cancer Research Institute, I-16132 Genova, Italy; M. Muselli: CNR-IEIIT, Genova, Italy; U. Pfeffer: Laboratory of Molecular Oncology, National Cancer Research Institute, I-16132 Genova, Italy.
Autori CNR e affiliazioni
  • MARCO MUSELLI
Lingua/e
  • inglese
AbstractThe 1785 nucleotides of the coding region of the estrogen receptor \alpha (ER-\alpha) are dispersed over a region of more than 300.000 nucleotides in the primary transcript. Splicing of this precursor RNA frequently leads to variants lacking one or more exons that have been associated to breast cancer progression. The most frequent splice variant lacks exon 4 and is expressed in the human mammary carcinoma cell line MCF-7 at a level similar to that of the full-length messenger. The in silico analysis of ER-\alpha splice sites by Hamming clustering, a self learning method trained on more than 28.000 experimentally proved splice sites, reveals high relevance for the 5' and 3' splice sites of exon 4. The splicing analysis of transfected mini-gene constructs containing drastically shortened introns excludes that weak splice sites, intron or exon lengths or splice enhancers are responsible for exon skipping. Exon 6 is never skipped in MCF-7 cells but is spliced out from mine-gene derived primary transcripts if inserted between exons 3 and 5 instead of exon 4. As a consequence, it appears that a particular splice site affinity of exon 3 donor and exon 5 acceptor sites is responsible for skipping of the exon in between.
Lingua abstractinglese
Altro abstract-
Lingua altro abstract-
Pagine da355
Pagine a363
Pagine totali9
RivistaInternational Journal of Molecular Medicine
Attiva dal 1998
Editore: D. A. Spandidos - Athens
Paese di pubblicazione: Grecia
Lingua: inglese
ISSN: 1107-3756
Titolo chiave: International Journal of Molecular Medicine
Titolo proprio: International Journal of Molecular Medicine.
Titolo abbreviato: Int. J. Mol. Med.
Numero volume della rivista12
Fascicolo della rivista3
DOI-
Verificato da refereeSì: Internazionale
Stato della pubblicazione-
Indicizzazione (in banche dati controllate)
  • ISI Web of Science (WOS) (Codice:000184475700011)
  • Scopus (Codice:2-s2.0-0642303089)
  • PubMed (Codice:12883652)
Parole chiaveestrogen receptor alpha, breast cancer, alternative splicing, mini-gene, Hamming clustering
Link (URL, URI)-
Titolo parallelo-
Data di accettazione-
Note/Altre informazioni-
Strutture CNR
  • IEIIT — Istituto di elettronica e di ingegneria dell'informazione e delle telecomunicazioni
Moduli CNR
    Progetti Europei-
    Allegati
    • Alternative splicing of the human estrogen receptor \alpha primary transcript: Mechanisms of exon skipping

    Dati storici
    I dati storici non sono modificabili, sono stati ereditati da altri sistemi (es. Gestione Istituti, PUMA, ...) e hanno solo valore storico.
    Rivista ISIINTERNATIONAL JOURNAL OF MOLECULAR MEDICINE [12412J0]