@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA969 pubblicazioni:autoreCNRDi prodotto:ID56888 . @prefix prodottidellaricerca: . @prefix modulo: . modulo:ID2205 prodottidellaricerca:prodotto prodotto:ID56888 . @prefix rdf: . @prefix retescientifica: . prodotto:ID56888 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID56888 rdfs:label "Mutation of a chloroplast targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long distance virus movement. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID56888 pubblicazioni:anno "2010-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID56888 skos:altLabel "
LIM H-S*; VAIRA AM*; BAE H; BRAGG JN; RUZIN SE; BAUCHAN GR; DIENELT MM; OWENS RA; HAMMOND J. (2010)
Mutation of a chloroplast targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long distance virus movement.
in Journal of general virology (Print)
"^^rdf:HTML ; pubblicazioni:autori "LIM H-S*; VAIRA AM*; BAE H; BRAGG JN; RUZIN SE; BAUCHAN GR; DIENELT MM; OWENS RA; HAMMOND J."^^xsd:string ; pubblicazioni:paginaInizio "2102"^^xsd:string ; pubblicazioni:paginaFine "2115"^^xsd:string ; pubblicazioni:numeroVolume "91"^^xsd:string . @prefix ns10: . prodotto:ID56888 pubblicazioni:rivista ns10:ID394986 ; pubblicazioni:note "Lim and Vaira hanno contribuito in modo uguale a questo lavoro"^^xsd:string ; pubblicazioni:descrizioneSinteticaDelProdotto "Publicazione sull\u0092importanza di un segnale di targeting ai cloroplasti, presente in una proteina virale nell\u0092efficacia del movimento del virus nella pianta, sia locale che sistemico."^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Hyoun-Sub Lim, USDA-ARS, USNA, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA\nHanhong Bae, USDA-ARS, PSI, Sustainable Perennial Crops Laboratory, Beltsville, MD 20705, USA\nJennifer N. Bragg, USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA\nSteven E. Ruzin, University of California-Berkeley, CNR, Berkeley, CA 94720, USA\nGary R. Bauchan, USDA-ARS, PSI, Electron and Confocal Microscopy Unit, Beltsville, MD 20705, USA\nMargaret M. Dienelt, USDA-ARS, USNA, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA\nRobert A. Owens, USDA-ARS, PSI, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA\nJohn Hammond, USDA-ARS, USNA, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA"^^xsd:string ; pubblicazioni:titolo "Mutation of a chloroplast targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long distance virus movement."^^xsd:string ; prodottidellaricerca:abstract "Cell-to-cell movement of potexviruses requires coordinated action of the coat protein and triple gene block (TGB) proteins. The structural properties of Alternanthera mosaic virus (AltMV) TGB3 were examined by methods differentiating between signal peptides and transmembrane domains, and its subcellular localization was studied by Agrobacterium-mediated transient expression and confocal microscopy. Unlike potato virus X (PVX) TGB3, AltMV TGB3 was not associated with the endoplasmic reticulum, and accumulated preferentially in mesophyll cells. Deletion and sitespecific mutagenesis revealed an internal signal VL(17,18) of TGB3 essential for chloroplast localization, and either deletion of the TGB3 start codon or alteration of the chloroplastlocalization signal limited cell-to-cell movement to the epidermis, yielding a virus that was unable to move into the mesophyll layer. Overexpression of AltMV TGB3 from either AltMV or PVX infectious clones resulted in veinal necrosis and vesiculation at the chloroplast membrane, a cytopathology not observed in wild-type infections. The distinctive mesophyll and chloroplast localization of AltMV TGB3 highlights the critical role played by mesophyll targeting in virus longdistance movement within plants."@en ; prodottidellaricerca:prodottoDi modulo:ID2205 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA969 . @prefix parolechiave: . prodotto:ID56888 parolechiave:insiemeDiParoleChiave . ns10:ID394986 pubblicazioni:rivistaDi prodotto:ID56888 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID56888 .