@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS081 prodottidellaricerca:prodotto prodotto:ID50182 . @prefix modulo: . modulo:ID2703 prodottidellaricerca:prodotto prodotto:ID50182 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA9690 pubblicazioni:autoreCNRDi prodotto:ID50182 . @prefix rdf: . prodotto:ID50182 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID50182 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID50182 rdfs:label "Antiproliferative activity of melatonin by transcriptional inhibition of cyclin D1 expression: a molecular basis for melatonin-induced oncostatic effects. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID50182 pubblicazioni:anno "2005-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID50182 skos:altLabel "
Cini G, Neri B, Pacini A, Cesati V, Sassoli C, Quattrone S, D'Apolito M, Fazio A, Scapagnini G, Provenzani A, Quattrone A. (2005)
Antiproliferative activity of melatonin by transcriptional inhibition of cyclin D1 expression: a molecular basis for melatonin-induced oncostatic effects.
in Journal of pineal research
"^^rdf:HTML ; pubblicazioni:autori "Cini G, Neri B, Pacini A, Cesati V, Sassoli C, Quattrone S, D'Apolito M, Fazio A, Scapagnini G, Provenzani A, Quattrone A."^^xsd:string ; pubblicazioni:paginaInizio "12"^^xsd:string ; pubblicazioni:paginaFine "20"^^xsd:string ; pubblicazioni:numeroVolume "39"^^xsd:string . @prefix ns11: . prodotto:ID50182 pubblicazioni:rivista ns11:ID454888 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Department of Pathology and Experimental Oncology, University of Florence, Florence, Italy.\n\n"^^xsd:string ; pubblicazioni:titolo "Antiproliferative activity of melatonin by transcriptional inhibition of cyclin D1 expression: a molecular basis for melatonin-induced oncostatic effects."^^xsd:string ; prodottidellaricerca:abstract "Melatonin is endowed with a growth inhibitory effect in MCF-7 breast cancer cells whose mechanism has been related to an antiestrogenic activity exerted by inhibition of binding of the estradiol-estrogen receptor complex to its DNA responsive element. Looking for downstream gene determinants of this effect, we performed a transcriptome profiling by high-density microarrays of estrogen-treated MCF-7 cells exposed or not to melatonin. We found that cyclin D1 was one of the main downregulated genes by melatonin. Validation experiments clearly confirm that in MCF-7 cells the estrogen-induced growth inhibitory activity of melatonin is consistently associated with inhibition of estrogen-elicited cyclin D1 induction. This effect is almost purely transcriptional. Reporter gene assays indicate that the same portion of the cyclin D1 promoter which confers estrogen sensitivity, encompassing a potential cAMP responsive element binding site, is repressed by melatonin. Transcriptional downregulation of cyclin D1 is the key molecular event for melatonin's antiproliferative activity, as this activity can be completely and selectively rescued by transient cyclin D1 overexpression. Finally, we provide indirect evidence that the effect of melatonin on the cyclin D1 promoter is mediated by the c-jun and ATF-2 proteins, known to bind the minimal estrogen-sensitive cyclin D1 promoter element. These findings establish for the first time a molecular link between melatonin and its effects on the cell cycle, providing at the same time a rationale for its use in adjuvant chemotherapy.\n\n" ; prodottidellaricerca:prodottoDi istituto:CDS081 , modulo:ID2703 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA9690 . ns11:ID454888 pubblicazioni:rivistaDi prodotto:ID50182 .