@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS080 prodottidellaricerca:prodotto prodotto:ID49059 . @prefix modulo: . modulo:ID3955 prodottidellaricerca:prodotto prodotto:ID49059 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA12518 pubblicazioni:autoreCNRDi prodotto:ID49059 . @prefix rdf: . prodotto:ID49059 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID49059 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID49059 rdfs:label "FISH mapping of 18S rDNA and (TTAGGG)(n) sequences in two pipefish species (Gasteroisteiformes : Syngnathidae). (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID49059 pubblicazioni:anno "2006-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID49059 skos:altLabel "
Libertini A. (1), Vitturi R. (2), Lannino A. (2), Maone MC. (2), Franzoi P. (3), Riccato F. (3), Colomba S. (4) (2006)
FISH mapping of 18S rDNA and (TTAGGG)(n) sequences in two pipefish species (Gasteroisteiformes : Syngnathidae).
in Journal of Genetics
"^^rdf:HTML ; pubblicazioni:autori "Libertini A. (1), Vitturi R. (2), Lannino A. (2), Maone MC. (2), Franzoi P. (3), Riccato F. (3), Colomba S. (4)"^^xsd:string ; pubblicazioni:paginaInizio "153"^^xsd:string ; pubblicazioni:paginaFine "156"^^xsd:string ; pubblicazioni:altreInformazioni "http://www.ias.ac.in/jgenet/Vol85number2.htm"^^xsd:string ; pubblicazioni:numeroVolume "85(2)"^^xsd:string . @prefix ns11: . prodotto:ID49059 pubblicazioni:rivista ns11:ID286613 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "(1)Istituto di Scienze Marine, Sezione di Venezia, CNR, Castello 1364/a, 30122 Venezia, Italy\n(2)Dipartimento di Biologia Animale, Universit`a di Palermo, Via Archirafi 18, 90123 Palermo, Italy\n(3)Dipartimento di Scienze Ambientali, Universit`a \u0093Ca\u0092 Foscari\u0094, Castello 2737/b - 30122 Venezia, Italy\n(4)Istituto di Ecologia e Biologia Ambientale, Universit`a di Urbino \u0093Carlo Bo\u0094, Via I. Maggetti 22,61029 Urbino (PU), Italy\n"^^xsd:string ; pubblicazioni:titolo "FISH mapping of 18S rDNA and (TTAGGG)(n) sequences in two pipefish species (Gasteroisteiformes : Syngnathidae)."^^xsd:string ; prodottidellaricerca:abstract "Chromosomal location of major ribosomal genes (18S\u00965.8S\u009628S rDNA) and (TTAGGG)n telomeric sequences has been investigated for the first time in pipefish (Syngnathidae) by application of fluorescence in situ hybridization (FISH). Two species were studied: Nerophis ophidion (L.) (2n karyotype = 58 metacentric, submetacentric and subtelocentric chromosomes)and Syngnathus abaster Risso (2n karyotype =44 acrocentric chromosomes) (Vitturi et al. 1998). The results indicate that in both pipefishes intraspecific numerical variation of ribosomal sites is more conspicuous than previously detected (Vitturi et al. 1998). (TTAGGG)n FISH\nshowed a regular hybridization pattern in S. abaster, while in N. ophidion it gave unexpected enlarged hybridization signals in the terminal region of either two or five chromosomes, where telomeric sequences were interspersed throughout nucleolus organizer regions NORs (interstitial telomeric sites, ITS). Diversification of ribosomal and telomeric repeats in the karyotype of the two species confirms the hypothesis that the genera Nerophis and Syngnathus have undergone two different pathways of karyotypic evolution.\nThe teleostean family Syngnathidae (pipefish and seahorses)includes 277 species, of which 11 inhabit the Mediterranean Sea and belong to the genera Nerophis (two species), Hippocampus (two species), Minyichthys (one\nspecies) and Syngnathus (six species) (FishBase 2005). Owing to the difficulty in obtaining high-quality chromosome preparations, cytogenetic data on syngnathids are scanty, referring in literature only to five Mediterranean species (two seahorses and three pipefishes), and are restricted to only a few aspects, including diploid chromosome number, karyotypic macrostructure, location of active NORs by silver staining, and genome size (Vitturi et al. 1998). With regard to NOR location, staining with silver nitrate and the fluorochrome chromomycin A3 (CMA3) has been widely applied to metaphase plates of both vertebrates and invertebrates to detect number and position of active NORs and\nGC-rich NORs, respectively (Sumner 1990, and references therein). However, both methods have proven to be inadequate\nin unequivocally characterizing the ribosomal pattern (active\nand inactive rDNA genes) in the karyotype of several species.\nIt has been shown that molecular techniques provide additional\nopportunities for animal cytogenetics.Gasteroisteiformes and its family Syngnathidae. In this note we report the first attempt to apply FISH to pipefish chromosomes.\n\n\n" ; prodottidellaricerca:prodottoDi istituto:CDS080 , modulo:ID3955 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA12518 . @prefix parolechiave: . prodotto:ID49059 parolechiave:insiemeDiParoleChiave . ns11:ID286613 pubblicazioni:rivistaDi prodotto:ID49059 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID49059 .