@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA22194 pubblicazioni:autoreCNRDi prodotto:ID46089 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS077 prodottidellaricerca:prodotto prodotto:ID46089 . @prefix modulo: . modulo:ID4270 prodottidellaricerca:prodotto prodotto:ID46089 . @prefix rdf: . @prefix retescientifica: . prodotto:ID46089 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID46089 rdfs:label "An electrochemical bioassay for dichlorvos analysis in durum wheat samples (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID46089 pubblicazioni:anno "2006-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID46089 skos:altLabel "
Del Carlo M.; Pepe A.; De Gregorio M.; Mascini M.; Marty J.L.; Fournier D.; Visconti A.; Compagnone D. (2006)
An electrochemical bioassay for dichlorvos analysis in durum wheat samples
in Journal of food protection
"^^rdf:HTML ; pubblicazioni:autori "Del Carlo M.; Pepe A.; De Gregorio M.; Mascini M.; Marty J.L.; Fournier D.; Visconti A.; Compagnone D."^^xsd:string ; pubblicazioni:paginaInizio "1406"^^xsd:string ; pubblicazioni:paginaFine "1411"^^xsd:string ; pubblicazioni:numeroVolume "69"^^xsd:string . @prefix ns11: . prodotto:ID46089 pubblicazioni:rivista ns11:ID348795 ; pubblicazioni:pagineTotali "6"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Department of Food Science, University of Teramo, 64023 Teramo, Italy.\nCentre de Phytopharmacie, Universit\u00E9 de Perpignan, 66860, Perpignan, France.\nLaboratoire de Synth\u00E8se et Phytochimie des Mol\u00E9cules d'Int\u00E9r\u00E8t Biologique, Universit\u00E9 Paul Sabatier, Tolouse, France.\nInstitute of Sciences of Food Production ISPA-CNR, 70126 Bari, Italy."^^xsd:string ; pubblicazioni:titolo "An electrochemical bioassay for dichlorvos analysis in durum wheat samples"^^xsd:string ; prodottidellaricerca:abstract "The use of an acetylcholinesterase inhibition assay for the detection of dichlorvos in durum wheat samples by a simplified extraction procedure is reported. After an incubation step, the residual activity was determined with an amperometric biosensor using a portable potentiostat. The use of electric eel and recombinant acetylcholinesterase was compared. The effect of the matrix extract was evaluated by using various sample:solvent ratios, 1:2.5, 1:5, 1:10, and 1:20. The optimal extraction ratio, considering the electrochemical interferences and the effect on enzyme activity and bioavailability of the pesticide, was 1:10. Calibrations were performed in buffer and durum wheat extract. The calculated detection limits in buffer solution were 10 ng/ml and 0.045 ng/ml for electric eel and recombinant acetylcholinesterase, respectively, whereas operating in the matrix extract they increased up to 45 ng/ml and 0.07 ng/ml, corresponding to 0.45 mg/kg (extraction ratio 1:10) and 0.07 mg/kg in samples. These characteristics allowed the detection of contaminated samples at the maximum residue limit, which is 2 mg/kg and well below. Fortified samples of durum wheat were obtained with both dichlorvos and the commercial product Didivane, which contains dichlorvos as an active molecule. At all the tested levels, the occurrence of contaminant was detected with an average recovery of 75%. The total assay time, including the extraction step, was 30 min. Because several extractions as well as most of the assay steps can be run simultaneously, the throughput for one operator is 12 determinations per hour."@en ; prodottidellaricerca:prodottoDi istituto:CDS077 , modulo:ID4270 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA22194 . @prefix parolechiave: . prodotto:ID46089 parolechiave:insiemeDiParoleChiave . ns11:ID348795 pubblicazioni:rivistaDi prodotto:ID46089 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID46089 .