@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS077 prodottidellaricerca:prodotto prodotto:ID46020 . @prefix modulo: . modulo:ID2228 prodottidellaricerca:prodotto prodotto:ID46020 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA9184 pubblicazioni:autoreCNRDi prodotto:ID46020 . @prefix rdf: . @prefix retescientifica: . prodotto:ID46020 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID46020 rdfs:label "Effect of proteinases of starter bacteria on the growth and proteolytic activity of Lactobacillus plantarum DPC2741 (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID46020 pubblicazioni:anno "2003-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID46020 skos:altLabel "
Di Cagno R., De Angelis M., Upadhyay V. K., McSweeney P.L.H., Minervini F., Gallo G., Gobbetti M. (2003)
Effect of proteinases of starter bacteria on the growth and proteolytic activity of Lactobacillus plantarum DPC2741
in International dairy journal
"^^rdf:HTML ; pubblicazioni:autori "Di Cagno R., De Angelis M., Upadhyay V. K., McSweeney P.L.H., Minervini F., Gallo G., Gobbetti M."^^xsd:string ; pubblicazioni:paginaInizio "145"^^xsd:string ; pubblicazioni:paginaFine "157"^^xsd:string ; pubblicazioni:numeroVolume "13"^^xsd:string . @prefix ns11: . prodotto:ID46020 pubblicazioni:rivista ns11:ID263373 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:titolo "Effect of proteinases of starter bacteria on the growth and proteolytic activity of Lactobacillus plantarum DPC2741"^^xsd:string ; prodottidellaricerca:abstract "The effects of partially purified proteinases (PrtP) of Streptococcus thermophilus ST8, Lactobacillus helveticus PR4, Lb. delbrueckii subsp. bulgaricus B397, Lactococcus lactis subsp. cremoris ST2 or Brevibacterium linens 9 on the growth and proteolytic activity of Lb. plantarum DPC2741 were studied. In sterile reconstituted skim milk (RSM), almost all the PrtP promoted higher cell numbers compared with the pure bacterial culture. The enzymes of Lb. helveticus PR4 and Lc. lactis subsp. cremoris ST2 were the most effective in increasing the maximum growth rate ( ), the time in which was held constant and in decreasing the lag phase of growth. Compared with the pure bacterial culture, higher concentrations of free amino acids were found when Lb. plantarum DPC2741 was cultivated in RSM with the PrtP of starter bacteria; the profiles of free amino acids and peptides depended on the type of PrtP. Lb. plantarum DPC2741 did not grow when -, -, or -casein (CN) fractions were substituted for amino acids in the chemically defined medium (CDM). When PrtP were added to CDM, bacterial growth was restored to an extent that depended on the substrate specificity of the enzyme and CN fraction used. Experiments in which combinations of the three essential amino acids (Glu, Val and Ile) were added to CDM containing CN fractions made it possible to identify some amino acid deficiency which was responsible for the limited growth of Lb. plantarum DPC2741 in combination with PrtP of starter bacteria. To simulate cheese ripening conditions, Lb. plantarum DPC2741 alone and in combinations with PrtP of Str. thermophilus ST8, Lb. delbrueckii subsp. bulgaricus B397 or Lb. helveticus PR4 was grown into a model cows\u00B4 milk curd system for 10 d at 12\u00B0C. All the PrtP enhanced the bacterial growth, increased the concentration of free amino acids and promoted profiles of amino acids and peptides qualitatively different." ; prodottidellaricerca:prodottoDi istituto:CDS077 , modulo:ID2228 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA9184 . ns11:ID263373 pubblicazioni:rivistaDi prodotto:ID46020 .