@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA22471 pubblicazioni:autoreCNRDi prodotto:ID4598 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS059 prodottidellaricerca:prodotto prodotto:ID4598 . @prefix modulo: . modulo:ID2651 prodottidellaricerca:prodotto prodotto:ID4598 . @prefix rdf: . prodotto:ID4598 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID4598 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID4598 rdfs:label "The metabotropic P2Y4 receptor participates in the commitment to differentiation and cell death of human neuroblastoma SH-SY5Y cells. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID4598 pubblicazioni:anno "2005-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1016/j.nbd.2004.09.001"^^xsd:string . @prefix skos: . prodotto:ID4598 skos:altLabel "
Cavaliere F.; Nestola V.2; Amadio S.3; D'Ambrosi N.4; Angelini D.F.5; Sancesario G.6; Bernardi G.7; Volont\u00E8 C.8 (2005)
The metabotropic P2Y4 receptor participates in the commitment to differentiation and cell death of human neuroblastoma SH-SY5Y cells.
in Neurobiology of disease
"^^rdf:HTML ; pubblicazioni:autori "Cavaliere F.; Nestola V.2; Amadio S.3; D'Ambrosi N.4; Angelini D.F.5; Sancesario G.6; Bernardi G.7; Volont\u00E8 C.8"^^xsd:string ; pubblicazioni:paginaInizio "100"^^xsd:string ; pubblicazioni:paginaFine "109"^^xsd:string ; pubblicazioni:altreInformazioni "IF =4.048 SCI-JCR 2005"^^xsd:string ; pubblicazioni:numeroVolume "18"^^xsd:string . @prefix ns11: . prodotto:ID4598 pubblicazioni:rivista ns11:ID224969 ; skos:note "ISI Web of Science (WOS)"^^xsd:string , "PubMe"^^xsd:string , "Scopu"^^xsd:string ; pubblicazioni:affiliazioni "1,2,3,4,6,7,8 =Fondazione Santa Lucia, Neurobiology Unit, Rome, Italy;\n3,4,6,7 =Department of Neuroscience, University of Rome Tor Vergata, Rome, Italy;\n5 =Fondazione Santa Lucia, Neuroimmunology Unit, Rome, Italy;\n8 =C.N.R. Institute of Neurobiology and Molecular Medicine, Rome, Italy."^^xsd:string ; pubblicazioni:titolo "The metabotropic P2Y4 receptor participates in the commitment to differentiation and cell death of human neuroblastoma SH-SY5Y cells."^^xsd:string ; prodottidellaricerca:abstract "Extracellular nucleotides exert a variety of biological actions through different subtypes of P2 receptors. Here we characterized in the human neuroblastoma SH-SY5Y cells the simultaneous presence of various P2 receptors, belonging to the P2X ionotropic and P2Y metabotropic families. Western blot analysis detected the P2X1,2,4,5,6,7 and P2Y1,2,4,6, but not the P2X3 and P2Y12 receptors. We then investigated which biological effects were mediated by the P2Y4 subtype and its physiological pyrimidine agonist UTP. We found that neuronal differentiation of the SH-SY5Y cells with dibutiryl-cAMP increased the expression of the P2Y4 protein and that UTP itself was able to positively interfere with neuritogenesis. Moreover, transient transfection and activation of P2Y4 also facilitated neuritogenesis in SH-SY5Y cells, as detected by morphological phase contrast analysis and confocal examination of neurofilament proteins NFL. This was concurrent with increased transcription of immediate-early genes linked to differentiation such as cdk-5 and NeuroD6, and activity of AP-1 transcription family members such as c-fos, fos-B, and jun-D. Nevertheless, a prolonged activation of the P2Y4 receptor by UTP also induced cell death, both in naive, differentiated, and P2Y4-transfected SH-SY5Y cells, as measured by direct count of intact nuclei and cytofluorimetric analysis of damaged DNA. Taken together, our data indicate that the high expression and activation of the P2Y4 receptor participates in the neuronal differentiation and commitment to death of SH-SY5Y cells."@en ; prodottidellaricerca:prodottoDi istituto:CDS059 , modulo:ID2651 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA22471 . @prefix parolechiave: . prodotto:ID4598 parolechiave:insiemeDiParoleChiave . ns11:ID224969 pubblicazioni:rivistaDi prodotto:ID4598 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID4598 .