@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS076 prodottidellaricerca:prodotto prodotto:ID45903 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA705 pubblicazioni:autoreCNRDi prodotto:ID45903 . @prefix modulo: . modulo:ID2629 prodottidellaricerca:prodotto prodotto:ID45903 . @prefix rdf: . prodotto:ID45903 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID45903 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID45903 rdfs:label "Bowman-Birk inhibitors in lentil: Heterologous expression, functional (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID45903 pubblicazioni:anno "2010-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID45903 skos:altLabel "
Caccialupi P, Ceci LR, Siciliano RA, Pignone D, Clemente A, Sonnante G. (2010)
Bowman-Birk inhibitors in lentil: Heterologous expression, functional
in Food chemistry
"^^rdf:HTML ; pubblicazioni:autori "Caccialupi P, Ceci LR, Siciliano RA, Pignone D, Clemente A, Sonnante G."^^xsd:string ; pubblicazioni:paginaInizio "1058"^^xsd:string ; pubblicazioni:paginaFine "1066"^^xsd:string ; pubblicazioni:numeroVolume "120"^^xsd:string . @prefix ns11: . prodotto:ID45903 pubblicazioni:rivista ns11:ID339802 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Institute of Plant Genetics, CNR, Via Amendola, 165/A, 70126 Bari, Italy\nInstitute for Biomembranes and Bioenergetics, CNR, Via Amendola, 165/A, 70126 Bari, Italy\nInstitute of Food Science, CNR, Via Roma, 64, 83100 Avellino, Italy\nEstaci\u00F3n Experimental del Zaid\u00EDn, CSIC, Profesor Albareda 1, 18008 Granada, Spain"^^xsd:string ; pubblicazioni:titolo "Bowman-Birk inhibitors in lentil: Heterologous expression, functional"^^xsd:string ; prodottidellaricerca:abstract "A full-length cDNA, encoding a Bowman-Birk protease inhibitor (BBI), was isolated from lentil immature\nseeds. The deduced amino acid sequence was longer than that of the BBI extracted from lentil seeds and\ncontained two binding sites; the first inhibitory site inhibits trypsin whereas the second one inhibits chymotrypsin.\nIn order to characterize this lentil BBI, a longer (complete) and its C-terminally processed\n(mature) form were heterologously expressed in the yeast Pichia pastoris. The recombinant BBI proteins\nproved to be active against trypsin and chymotrypsin, showing Ki values at nanomolar levels. Mass spectrometry\nanalysis revealed that complete BBI was composed of an array of molecular masses, whereas\nmature BBI showed the presence of a major peak of the expected size. The effects of mature BBI on the\ngrowth of human colon adenocarcinoma HT29 and colonic fibroblast CCD-18Co cells were evaluated.\nLentil BBI was able to inhibit the growth of such cells at concentrations higher than 19 lM, in a concentration-\ndependent manner; by contrast, the CCD18-Co cells were unaffected. These data broaden our\nknowledge of the beneficial biological activities of naturally-occurring BBI proteins and address the need\nfor systematic evaluation of natural variants in order to design novel strategies in preventive medicine." ; prodottidellaricerca:prodottoDi istituto:CDS076 , modulo:ID2629 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA705 . ns11:ID339802 pubblicazioni:rivistaDi prodotto:ID45903 .