@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA39385 pubblicazioni:autoreCNRDi prodotto:ID4485 . unitaDiPersonaleInterno:MATRICOLA6655 pubblicazioni:autoreCNRDi prodotto:ID4485 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS059 prodottidellaricerca:prodotto prodotto:ID4485 . @prefix modulo: . modulo:ID2572 prodottidellaricerca:prodotto prodotto:ID4485 . @prefix rdf: . @prefix retescientifica: . prodotto:ID4485 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID4485 rdfs:label "Telomerase mediates vascular endothelial growth factor-dependent responsiveness in a rat model of hind limb ischemia (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID4485 pubblicazioni:anno "2005-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID4485 skos:altLabel "
Zaccagnini G.1; Gaetano C.2; Della Pietra L.3; Nanni S.4; Grasselli A.5; Mangoni A.6; Benvenuto R.7; Fabrizi M.8; Truffa S.9; Germani A.10; Moretti F.11; Pontecorvi A.12; Sacchi A.13; Bacchetti S.14; Capogrossi M.C.15; Farsetti A.16 (2005)
Telomerase mediates vascular endothelial growth factor-dependent responsiveness in a rat model of hind limb ischemia
in The Journal of biological chemistry (Print)
"^^rdf:HTML ; pubblicazioni:autori "Zaccagnini G.1; Gaetano C.2; Della Pietra L.3; Nanni S.4; Grasselli A.5; Mangoni A.6; Benvenuto R.7; Fabrizi M.8; Truffa S.9; Germani A.10; Moretti F.11; Pontecorvi A.12; Sacchi A.13; Bacchetti S.14; Capogrossi M.C.15; Farsetti A.16"^^xsd:string ; pubblicazioni:paginaInizio "14790"^^xsd:string ; pubblicazioni:paginaFine "14798"^^xsd:string ; pubblicazioni:altreInformazioni "Impact Factor = 5.328"^^xsd:string ; pubblicazioni:numeroVolume "280"^^xsd:string . @prefix ns11: . prodotto:ID4485 pubblicazioni:rivista ns11:ID381209 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "1,6,10,11,16 = Laboratory of Vascular Biology and Gene Therapy, Cardiology Center \\\"I. Monzino,\\\" Milan 20138, Italy;\n2,7,8,9,15,16 = Laboratory of Vascular Pathology, Istituto Dermopatico dell'Immacolata, Rome 00167, Italy;\n3,5,11,12,13,14 = Department of Experimental Oncology, Molecular Oncogenesis Laboratory, Regina Elena Institute-Centro Ricerche Sperimentali, Rome 00158, Italy;\n12 = Institute of Medical Pathology, Catholic University, Rome 00168, Italy;\n11, 16 = Institute of Neurobiology and Molecular Medicine, National Research Council, Rome 00137, Italy."^^xsd:string ; pubblicazioni:titolo "Telomerase mediates vascular endothelial growth factor-dependent responsiveness in a rat model of hind limb ischemia"^^xsd:string ; prodottidellaricerca:abstract "Telomere dysfunction contributes to reduced cell viability, altered differentiation, and impaired regenerative/proliferative responses. Recent advances indicate that telomerase activity confers a pro-angiogenic phenotype to endothelial cells and their precursors. We have investigated whether telomerase contributes to tissue regeneration following hind limb ischemia and vascular endothelial growth factor 165 (VEGF(165)) treatment. VEGF delivery induced angiogenesis and increased expression of the telomerase reverse transcriptase (TERT) and telomerase activity in skeletal muscles and satellite and endothelial cells. Adenovirus-mediated transfer of wild type TERT but not of a dominant negative mutant, TERTdn, significantly induced capillary but not arteriole formation. However, when co-delivered with VEGF, TERTdn abrogated VEGF-dependent angiogenesis, arteriogenesis, and blood flow increase. This effect was paralleled by in vitro evidence that telomerase inhibition by 3'-azido-3'-deoxythymidine in VEGF-treated endothelial cells strongly reduced capillary density and promoted apoptosis in the absence of serum. Similar results were obtained with adenovirus-mediated expression of TERTdn and AKTdn, both reducing endogenous TERT activity and angiogenesis on Matrigel. Mechanistically, neo-angiogenesis in our system involved: (i) VEGF-dependent activation of telomerase through the nitric oxide pathway and (ii) telomerase-dependent activation of endothelial cell differentiation and protection from apoptosis. Furthermore, detection of TERT in activated satellite cells identified them as VEGF targets during muscle regeneration. Because TERT behaves as an angiogenic factor and a downstream effector of VEGF signaling, telomerase activity appears required for VEGF-dependent remodeling of ischemic tissue at the capillaries and arterioles level." ; prodottidellaricerca:prodottoDi istituto:CDS059 , modulo:ID2572 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA39385 , unitaDiPersonaleInterno:MATRICOLA6655 . ns11:ID381209 pubblicazioni:rivistaDi prodotto:ID4485 .