@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA22471 pubblicazioni:autoreCNRDi prodotto:ID4432 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS059 prodottidellaricerca:prodotto prodotto:ID4432 . @prefix rdf: . @prefix retescientifica: . prodotto:ID4432 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID4432 rdfs:label "2-ChloroATP exerts antitumoral actions not mediated by P2 receptors in neuronal and glial cell lines. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID4432 pubblicazioni:anno "2004-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1016/j.bcp.2003.09.015"^^xsd:string . @prefix skos: . prodotto:ID4432 skos:altLabel "
D'Ambrosi N.1, Costanzi S.2, Angelini D.F.3, Volpini R.4, Sancesario G.5, Cristalli G.6, Volont\u00E9 C.7 (2004)
2-ChloroATP exerts antitumoral actions not mediated by P2 receptors in neuronal and glial cell lines.
in Biochemical pharmacology
"^^rdf:HTML ; pubblicazioni:autori "D'Ambrosi N.1, Costanzi S.2, Angelini D.F.3, Volpini R.4, Sancesario G.5, Cristalli G.6, Volont\u00E9 C.7"^^xsd:string ; pubblicazioni:paginaInizio "621"^^xsd:string ; pubblicazioni:paginaFine "630"^^xsd:string ; pubblicazioni:altreInformazioni "IF =3.436 SCI-JCR 2004"^^xsd:string ; pubblicazioni:numeroVolume "67"^^xsd:string . @prefix ns10: . prodotto:ID4432 pubblicazioni:rivista ns10:ID293154 ; pubblicazioni:numeroFascicolo "4"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string , "Scopu"^^xsd:string , "PubMe"^^xsd:string ; pubblicazioni:affiliazioni "1,7 = Fondazione Santa Lucia, Cellular Neurobiology Unit, Rome, Italy;\n1,5 = Department of Neuroscience, University of Rome Tor Vergata, Rome, \n Rome, Italy;\n2,4,6 = Department of Chemical Sciences, University of Camerino, Camerino\n Italy;\n3 = Fondazione Santa Lucia, Neuroimmunology Unit, Rome, Italy;\n7 = C.N.R., INMM, Rome, Italy."^^xsd:string ; pubblicazioni:titolo "2-ChloroATP exerts antitumoral actions not mediated by P2 receptors in neuronal and glial cell lines."^^xsd:string ; prodottidellaricerca:abstract "We investigated the effects of the ATP analogue and P2 receptor agonist 2-ClATP on growth and survival of different neuronal (PC12, PC12nnr5 and SH-SY5Y) and glial (U87 and U373) cell lines, by the use of direct count of intact nuclei, fluorescence microscopy, fluorescence-activated cell sorter analysis (FACS) and high pressure liquid chromatography (HPLC). 2-ClATP lowered the number of cultured PC12nnr5, SH-SY5Y, U87 and U373 cells to almost 5%, and of PC12 cells to about 35% after 3-4 days of treatment. EC(50) was in the 5-25 microM range, with 2-ClATP behaving as a cytotoxic or cytostatic agent. Analysis of the biological mechanisms demonstrated that pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (P2 receptor antagonist and nucleotidases inhibitor), but not Caffeine or CGS-15493 (P1 receptor antagonists) effectively prevented 2-ClATP-induced toxicity. 2-ClATP metabolic products (2-ClADP, 2-ClAMP, 2-Cladenosine) and new synthesis derivatives (2-CldAMP, 2-Cldadenosine-3',5'-bisphosphate and 2-CldATP) exerted similar cytotoxic actions. Inhibition of both serum nucleotidases and purine nucleoside transporters strongly reduced 2-ClATP-induced cell death, which was conversely increased by the nucleotide hydrolyzing enzyme apyrase. The adenosine kinase inhibitor 5-iodotubericidin totally prevented 2-ClATP or 2-Cladenosine-induced toxicity. In summary, our findings indicate that 2-ClATP exerts either cell cycle arrest or cell death, acting neither on P2 nor on P1 receptors, but being extracellularly metabolized into 2-Cladenosine, intracellularly transported and re-phosphorylated."@en ; prodottidellaricerca:prodottoDi istituto:CDS059 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA22471 . @prefix parolechiave: . prodotto:ID4432 parolechiave:insiemeDiParoleChiave . ns10:ID293154 pubblicazioni:rivistaDi prodotto:ID4432 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID4432 .