@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS069 prodottidellaricerca:prodotto prodotto:ID42293 . @prefix modulo: . modulo:ID3951 prodottidellaricerca:prodotto prodotto:ID42293 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA22113 pubblicazioni:autoreCNRDi prodotto:ID42293 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID3936 pubblicazioni:autoreCNRDi prodotto:ID42293 . @prefix rdf: . @prefix retescientifica: . prodotto:ID42293 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID42293 rdfs:label "ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID42293 pubblicazioni:anno "2008-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID42293 skos:altLabel "
VIGAN\u00D2 L., BENFENATI E., CAUWENBERGE A., EIDEM J., ERRATICO C., GOKSOYR A., KLOAS W., MAGGIONI S., MANDICH A., URBATZKA R. (2008)
ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS
in Chemosphere
"^^rdf:HTML ; pubblicazioni:autori "VIGAN\u00D2 L., BENFENATI E., CAUWENBERGE A., EIDEM J., ERRATICO C., GOKSOYR A., KLOAS W., MAGGIONI S., MANDICH A., URBATZKA R."^^xsd:string ; pubblicazioni:paginaInizio "1078"^^xsd:string ; pubblicazioni:paginaFine "1089"^^xsd:string ; pubblicazioni:numeroVolume "73"^^xsd:string . @prefix ns12: . prodotto:ID42293 pubblicazioni:rivista ns12:ID305285 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Water Research Institute, National Council of Research, Brugherio (Milan), Italy\nMario Negri Institute, Laboratory of Environmental Chemistry and Toxicology, Milan, Italy\nInstitut Provincial d'Hygi\u00E9ne et de Bact\u00E9riologie, Mons, Belgium\nBiosense Laboratories AS, Bergen, Norway Department of Molecular Biology, University of Bergen, Bergen, Norway\nDepartment of Inland Fisheries, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany\nDepartment of Endocrinology, Institute of Biology, Humboldt-University, Berlin, Germany\nDepartment of Environmental, Experimental and Applied Biology, University of Genoa, Genoa, Italy"^^xsd:string ; pubblicazioni:titolo "ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS"^^xsd:string ; prodottidellaricerca:abstract "An effects-directed strategy was applied to bed sediments of a polluted tributary in order to isolate and\nidentify the major estrogenic chemicals it discharges into the River Po, the principal Italian watercourse.\nSediment extract was concentrated by solid phase extraction and then fractioned into 10 fractions by\nreversed phase high performance liquid chromatography (RP-HPLC). Estrogenic activity of whole extract\nand fractions were determined using a recombinant yeast assay containing the human estrogen receptor\n(YES). The 10 fractions and whole extract were analysed for target compounds, e.g. estrone (E1), 17bestradiol\n(E2), estriol (E3), 4-nonylphenol (NP), 4-tert-octylphenol (t-OP), bisphenol A (BPA), using both\nliquid chromatography-tandem mass spectrometry (LC-MS/MS) and non-competitive enzyme-linked\nimmunosorbent assays (ELISA). The YES assay determined high estrogenic activity in whole sediment\n(15.6 ng/g EE2 equivalents), and positive results for fractions nr 1, 2, 6, 7 and 8. E1, E3 and NP were\nthe main estrogenic chemicals, however, other unidentified compounds contributed to sediment estrogenicity,\nparticularly for polar fractions nr 1 and 2. A GC-MS screening performed in scan mode identified\nother potential contributors such as phthalates (DBP, BBP), and OP isomers. A next sampling campaign\nextended to other tributaries and receiving stretches of the River Po confirmed E1, E3 and NP as major\nestrogenic chemicals potentially threatening other sites of the main river. In general, target compound\nELISAs have been shown to be suitable tools for a rapid screening of wide areas or large numbers of environmental\nsamples for estrogenic risk. The potential for interferences suggests however to use cautiously\nthe concentration values obtained from some of the immunoassays."@en ; prodottidellaricerca:prodottoDi modulo:ID3951 , istituto:CDS069 ; pubblicazioni:autoreCNR unitaDiPersonaleEsterno:ID3936 , unitaDiPersonaleInterno:MATRICOLA22113 . ns12:ID305285 pubblicazioni:rivistaDi prodotto:ID42293 .