@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS064 prodottidellaricerca:prodotto prodotto:ID41213 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA8556 pubblicazioni:autoreCNRDi prodotto:ID41213 . @prefix modulo: . modulo:ID5881 prodottidellaricerca:prodotto prodotto:ID41213 . @prefix rdf: . prodotto:ID41213 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID41213 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID41213 rdfs:label "Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID41213 pubblicazioni:anno "2004-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1111/j.1365-313X.2004.02162.x"^^xsd:string . @prefix skos: . prodotto:ID41213 skos:altLabel "
Guyon V., Tang W.H., Monti M.M., Raiola A., Lorenzo G.D., McCormick S., Taylor L.P. (2004)
Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth.
in Plant journal (Print)
"^^rdf:HTML ; pubblicazioni:autori "Guyon V., Tang W.H., Monti M.M., Raiola A., Lorenzo G.D., McCormick S., Taylor L.P."^^xsd:string ; pubblicazioni:paginaInizio "643"^^xsd:string ; pubblicazioni:paginaFine "654"^^xsd:string ; pubblicazioni:numeroVolume "39"^^xsd:string . @prefix ns11: . prodotto:ID41213 pubblicazioni:rivista ns11:ID263471 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "-Guyon V., Taylor L.P., School of Molecular Biosciences, Washington State University, Pullman, WA 99163-4234, USA\n- Tang W.H., McCormick S., Plant Gene Expression Center, USDA/ARS and Department of Plant and Microbial Biology, University of California, Berkeley, CA 94710, USA\n- Raiola A., Lorenzo G.D., Department of Plant Biology, University La Sapienza, 00185 Rome, Italy\n- Monti M.M., Istituto per la Protezione delle Piante, CNR, Portici,Via Universit\u00E0 133, 80055, Portici, Napoli, Italy"^^xsd:string ; pubblicazioni:titolo "Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth."^^xsd:string ; prodottidellaricerca:abstract "SHY, a pollen-specific gene identified in a screen for genes upregulated at pollen germination (Guyon et al., 2000), encodes a leucine-rich repeat (LRR) protein that is predicted to be secreted. To test if SHY plays an important role during pollen germination, we generated transgenic plants expressing an antisense (AS) copy of the SHY cDNA in pollen. Primary transformants exhibited poor seed set, but homozygous lines could be identified. In these lines, nearly all pollen tubes failed to reach the ovules; tube growth was arrested at the apex of the ovary and the pollen tubes exhibited abnormal callose deposits throughout the tube and in the tips. We show that a SHY::eGFP fusion protein is targeted to the cell wall. The structure of the SHY protein is nearly identical to other extracellular matrix glycoproteins that are composed of LRRs, such as the polygalacturonase inhibitor proteins (PGIP) of plants. PGIPs may function as defense proteins by inhibiting fungal endo-polygalacturonases, but enzyme assays with extracts of AS-SHY pollen do not support such an inhibitor role for SHY. The tomato ortholog of SHY interacts with a tomato receptor kinase (LePRK2) in yeast two-hybrid and pull-down assays (Tang et al., 2002, 2004); this, and the AS-SHY phenotypes, suggest instead that SHY might function in a signal transduction pathway mediating pollen tube growth."@en ; prodottidellaricerca:prodottoDi istituto:CDS064 , modulo:ID5881 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA8556 . @prefix parolechiave: . prodotto:ID41213 parolechiave:insiemeDiParoleChiave . ns11:ID263471 pubblicazioni:rivistaDi prodotto:ID41213 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID41213 .