@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA5372 pubblicazioni:autoreCNRDi prodotto:ID37511 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS061 prodottidellaricerca:prodotto prodotto:ID37511 . @prefix rdf: . @prefix retescientifica: . prodotto:ID37511 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID37511 rdfs:label "Glutamate-mediated cytosolic calcium oscillations regulate a pulsatile prostaglandin release from cultured rat astrocytes. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID37511 pubblicazioni:anno "2003-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID37511 skos:altLabel "
Zonta M., Sebelin A., Gobbo S., Fellin T., Pozzan T., Carmignoto G. (2003)
Glutamate-mediated cytosolic calcium oscillations regulate a pulsatile prostaglandin release from cultured rat astrocytes.
"^^rdf:HTML ; pubblicazioni:autori "Zonta M., Sebelin A., Gobbo S., Fellin T., Pozzan T., Carmignoto G."^^xsd:string ; pubblicazioni:paginaInizio "407"^^xsd:string ; pubblicazioni:paginaFine "414"^^xsd:string ; pubblicazioni:altreInformazioni "impact factor 4,352"^^xsd:string ; pubblicazioni:numeroVolume "553"^^xsd:string ; pubblicazioni:descrizioneSinteticaDelProdotto "pubblicazione su rivista internazionale"^^xsd:string ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Uni di PD, Dipartimento di Scienze Biomediche Sperimentali"^^xsd:string ; pubblicazioni:titolo "Glutamate-mediated cytosolic calcium oscillations regulate a pulsatile prostaglandin release from cultured rat astrocytes."^^xsd:string ; prodottidellaricerca:abstract "The synaptic release of glutamate evokes in astrocytes periodic increases in [Ca2+]i, due to the activation of metabotropic glutamate receptors (mGluRs). The frequency of these [Ca2+]i oscillations is controlled by the level of neuronal activity, indicating that they represent a specific, frequency-coded signalling system of neuron-to-astrocyte communication. We recently found that neuronal activity-dependent [Ca2+]i oscillations in astrocytes are the main signal that regulates the coupling between neuronal activity and blood flow, the so-called functional hyperaemia. Prostaglandins play a major role in this fundamental phenomenon in brain function, but little is known about a possible link between [Ca2+]i oscillations and prostaglandin release from astrocytes. To investigate whether [Ca2+]i oscillations regulate the release of vasoactive prostaglandins, such as the potent vasodilator prostaglandin E2 (PGE2), from astrocytes, we plated wild-type human embryonic kidney (HEK)293 cells, which respond constitutively to PGE2 with [Ca2+]i elevations, onto cultured astrocytes, and used them as biosensors of prostaglandin release. After loading the astrocyte-HEK cell co-cultures with the calcium indicator Indo-1, confocal microscopy revealed that mGluR-mediated [Ca2+]i oscillations triggered spatially and temporally coordinated [Ca2+]i increases in the sensor cells. This response was absent in a clone of HEK cells that are unresponsive to PGE2, and recovered after transfection with the InsP3-linked prostanoid receptor EP1. We conclude that [Ca2+]i oscillations in astrocytes regulate prostaglandin releases that retain the oscillatory behaviour of the [Ca2+]i changes. This finely tuned release of PGE2 from astrocytes provides a coherent mechanistic background for the role of these glial cells in functional hyperaemia." ; prodottidellaricerca:prodottoDi istituto:CDS061 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA5372 . @prefix parolechiave: . prodotto:ID37511 parolechiave:insiemeDiParoleChiave . parolechiave:insiemeDiParoleChiaveDi prodotto:ID37511 .