@prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . @prefix prodotto: . unitaDiPersonaleInterno:MATRICOLA8493 pubblicazioni:autoreCNRDi prodotto:ID33108 . @prefix prodottidellaricerca: . @prefix istituto: . istituto:CDS053 prodottidellaricerca:prodotto prodotto:ID33108 . unitaDiPersonaleInterno:MATRICOLA6038 pubblicazioni:autoreCNRDi prodotto:ID33108 . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID3450 pubblicazioni:autoreCNRDi prodotto:ID33108 . @prefix rdf: . @prefix retescientifica: . prodotto:ID33108 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID33108 rdfs:label "Enantiomeric separation of mirtazapine and its metabolites by nano-Liquid chromatography with UV-absorption and mass spectrometric detection. (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID33108 pubblicazioni:anno "2005-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID33108 skos:altLabel "
S. Fanali, Z. Aturki, V. Kasicka, M. A. Raggi, G. D\u0092Orazio (2005)
Enantiomeric separation of mirtazapine and its metabolites by nano-Liquid chromatography with UV-absorption and mass spectrometric detection.
in Journal of separation science (Print)
"^^rdf:HTML ; pubblicazioni:autori "S. Fanali, Z. Aturki, V. Kasicka, M. A. Raggi, G. D\u0092Orazio"^^xsd:string ; pubblicazioni:paginaInizio "1719"^^xsd:string ; pubblicazioni:numeroVolume "28"^^xsd:string . @prefix ns11: . prodotto:ID33108 pubblicazioni:rivista ns11:ID58677 ; skos:note "ISI Web of Science (WOS)"^^xsd:string ; pubblicazioni:affiliazioni "Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche"^^xsd:string ; pubblicazioni:titolo "Enantiomeric separation of mirtazapine and its metabolites by nano-Liquid chromatography with UV-absorption and mass spectrometric detection."^^xsd:string ; prodottidellaricerca:abstract "Mirtazapine (MIR) and two of its main metabolites, namely, 8-hydroxymirtazapine and N-desmethylmirtazapine, were separated in totheir enantiomers by nanoLC in a laboratory-made fused-silica capillary column (75 micron ID) packed with a vancomycinmodified silica stationary phase. The simultaneous separation of the three couples of the studied enantiomers was achieved in less than 33 min, using an experimentally optimized mobile phase delivered in the isocratic mode. Optimization of the mobile phase\ncomposition was achieved by testing the influence of the buffer pH and concentration, the water concentration, the organic modifier type and concentration, and on the retention and resolution of the analytes. The optimum mobile-phase composition contained 500 mM ammonium acetate pH 4.5/water/MeOH/MeCN, 1:14:40:45 v/v/v/v. Using a UV detector at 205 nm, the method was validated studying several experimental parameters such as LOD and LOQ, intraday and interday repeatability, and linearity. Good results were achieved: LOD and LOQ were in the range 5\u009615 and 10\u009640 microg/mL, respectively (the highest value was obtained for the DEMIR enantiomers);\ncorrelation coefficients, 0.9993\u00960.9999; the intraday and interday precision was acceptable (RSD a 2%) using an internal standard. The method was tested for the separation of the studied enantiomers in an extracted (solid-phase) serum sample spiked with standard racemic mixture of MIR and its two metabolites. Finally, the nanoLC system was connected to a mass spectrometer through a nanoelectrospray interface and the MS, MS2, and MS3 spectra were acquired showing the potential of the system used for characterization and identification of the separated analytes." ; prodottidellaricerca:prodottoDi istituto:CDS053 ; pubblicazioni:autoreCNR unitaDiPersonaleEsterno:ID3450 , unitaDiPersonaleInterno:MATRICOLA6038 , unitaDiPersonaleInterno:MATRICOLA8493 . @prefix parolechiave: . prodotto:ID33108 parolechiave:insiemeDiParoleChiave . ns11:ID58677 pubblicazioni:rivistaDi prodotto:ID33108 . parolechiave:insiemeDiParoleChiaveDi prodotto:ID33108 .