@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS009 prodottidellaricerca:prodotto prodotto:ID328938 . @prefix pubblicazioni: . @prefix unitaDiPersonaleEsterno: . unitaDiPersonaleEsterno:ID10422 pubblicazioni:autoreCNRDi prodotto:ID328938 . @prefix modulo: . modulo:ID7742 prodottidellaricerca:prodotto prodotto:ID328938 . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA14867 pubblicazioni:autoreCNRDi prodotto:ID328938 . unitaDiPersonaleEsterno:ID16302 pubblicazioni:autoreCNRDi prodotto:ID328938 . @prefix rdf: . prodotto:ID328938 rdf:type prodotto:TIPO1101 . @prefix retescientifica: . prodotto:ID328938 rdf:type retescientifica:ProdottoDellaRicerca . @prefix rdfs: . prodotto:ID328938 rdfs:label "Proteomic analysis of human glioblastoma cell lines differently resistant to a nitric oxide releasing agent (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID328938 pubblicazioni:anno "2015-01-01T00:00:00+01:00"^^xsd:gYear ; pubblicazioni:doi "10.1039/c4mb00725e"^^xsd:string . @prefix skos: . prodotto:ID328938 skos:altLabel "
Roberta Leone a, Paola Giussani b, Sara De Palma c, Chiara Fania a, Daniele Capitanio a,d, Michele Vasso c, Loredana Brioschi b, Laura Riboni b, Paola Viani b and Cecilia Gelfi *a,c,d (2015)
Proteomic analysis of human glioblastoma cell lines differently resistant to a nitric oxide releasing agent
in Molecular bioSystems (Print)
"^^rdf:HTML ; pubblicazioni:autori "Roberta Leone a, Paola Giussani b, Sara De Palma c, Chiara Fania a, Daniele Capitanio a,d, Michele Vasso c, Loredana Brioschi b, Laura Riboni b, Paola Viani b and Cecilia Gelfi *a,c,d"^^xsd:string ; pubblicazioni:altreInformazioni "Pubblicato on-line: 23 Marzo 2015"^^xsd:string . @prefix ns12: . prodotto:ID328938 pubblicazioni:rivista ns12:ID99082 ; skos:note "Pubme"^^xsd:string ; pubblicazioni:affiliazioni "a. Department of Biomedical Sciences for Health, University of Milan, Segrate (MI), Italy. \nb. Department of Medical Biotechnology and Translational Medicine, University of Milan, Segrate (MI), Italy\nc. Institute of Molecular Bioimaging and Physiology (IBFM), CNR, Cefal\u00F9 (PA) Italy \nd. IRCCS Policlinico San Donato, Milan, Italy\n+ Electronic supplementary information (ESI) available."^^xsd:string ; pubblicazioni:titolo "Proteomic analysis of human glioblastoma cell lines differently resistant to a nitric oxide releasing agent"^^xsd:string ; prodottidellaricerca:abstract "Glioblastoma multiforme is the most aggressive astrocytoma characterized by the development of resistant cells to various cytotoxic stimuli. Nitric oxide (NO) is able to overcome tumor resistance in PTEN mutated rat C6 glioma cells due to its ability to inhibit cell growth by influencing the intracellular distribution of ceramide. The aim of this study is to monitor the effects of NO donor PAPANONOate on ceramide trafficking in human glioma cell lines, CCF-STTG1 (PTEN-mutated, p53-wt) and T98G (PTEN-harboring, p53-mutated), together with the assessment of their differential molecular signature by 2D-DIGE and MALDI mass spectrometry. In the CCF-STTG1 cell line, the results indicate that treatment with PAPANONOate decreased cell proliferation (<50%) and intracellular trafficking of ceramide, assessed by BODIPY-C5Cer, while these events were not observed in the T98G cell line. Proteomic results suggest that CCF-STTG1 cells are characterized by an increased expression of proteins involved in NO-associated ER stress (i.e. protein disulfide-isomerase A3, calreticulin, 78 kDa glucose-regulated protein), which could compromise ceramide delivery from ER to Golgi, leading to ceramide accumulation in ER and partial growth arrest. Conversely, T98G cell lines, resistant to NO exposure, are characterized by increased levels of cytosolic antioxidant proteins (i.e. glutathione-S-transferase P, peroxiredoxin 1), which might buffer intracellular NO. By providing differential ceramide distribution after NO exposure and differential protein expression of two high grade glioma cell lines, this study highlights specific proteins as possible markers for tumor aggressiveness. This study demonstrates that, in two different high grade glioma cell lines, NO exposure results in a different ceramide distribution and protein expression. Furthermore, this study highlights specific proteins as possible markers for tumor aggressiveness."@en ; prodottidellaricerca:prodottoDi istituto:CDS009 , modulo:ID7742 ; pubblicazioni:autoreCNR unitaDiPersonaleEsterno:ID10422 , unitaDiPersonaleEsterno:ID16302 , unitaDiPersonaleInterno:MATRICOLA14867 . ns12:ID99082 pubblicazioni:rivistaDi prodotto:ID328938 .