@prefix prodottidellaricerca: . @prefix istituto: . @prefix prodotto: . istituto:CDS040 prodottidellaricerca:prodotto prodotto:ID324541 . @prefix pubblicazioni: . @prefix unitaDiPersonaleInterno: . unitaDiPersonaleInterno:MATRICOLA5292 pubblicazioni:autoreCNRDi prodotto:ID324541 . @prefix rdf: . @prefix retescientifica: . prodotto:ID324541 rdf:type retescientifica:ProdottoDellaRicerca , prodotto:TIPO1101 . @prefix rdfs: . prodotto:ID324541 rdfs:label "Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers (Articolo in rivista)"@en . @prefix xsd: . prodotto:ID324541 pubblicazioni:anno "1991-01-01T00:00:00+01:00"^^xsd:gYear . @prefix skos: . prodotto:ID324541 skos:altLabel "
Bergamaschi, Gaetano M.; Carlo-Stella, Carmelo Stella; Cattoretti, Giorgio; Invernizzi, Rosangela; Maserati, Emanuela; Nalli, Giulio; Pedrazzoli, Paolo; Peverali, Fiorenzo A A; Della Valle, Giuliano; ASCARI, Edoardo; Cazzola, Mario (1991)
Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers
in British journal of haematology (Print)
"^^rdf:HTML ; pubblicazioni:autori "Bergamaschi, Gaetano M.; Carlo-Stella, Carmelo Stella; Cattoretti, Giorgio; Invernizzi, Rosangela; Maserati, Emanuela; Nalli, Giulio; Pedrazzoli, Paolo; Peverali, Fiorenzo A A; Della Valle, Giuliano; ASCARI, Edoardo; Cazzola, Mario"^^xsd:string ; pubblicazioni:paginaInizio "167"^^xsd:string ; pubblicazioni:paginaFine "172"^^xsd:string ; pubblicazioni:url "http://www.scopus.com/record/display.url?eid=2-s2.0-0025883279&origin=inward"^^xsd:string ; pubblicazioni:numeroVolume "78"^^xsd:string . @prefix ns10: . prodotto:ID324541 pubblicazioni:rivista ns10:ID293206 ; pubblicazioni:numeroFascicolo "2"^^xsd:string ; skos:note "Scopu"^^xsd:string ; pubblicazioni:affiliazioni "Fondazione IRCCS Policlinico San Matteo"^^xsd:string ; pubblicazioni:titolo "Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers"^^xsd:string ; prodottidellaricerca:abstract "We describe a novel continuous B-cell line (PV-90) derived from a patient with myelodysplastic syndrome (MDS) and originating from spontaneous infection with the Epstein-Barr virus (EBV). The patient progressed to acute myeloblastic leukaemia (AML) 5 months after clinical onset of MDS. PV-90 is of clonal origin as indicated by the presence of immunoglobulin (Ig) gene rearrangements, monoclonal surface immunoglobulins, and a single DNA restriction fragment corresponding to the EBV genomic termini. PV-90 cells also express a number of myelomonocytic markers, including ?-naphthyl acetate esterase (ANAE), coagulation factor XIII, and CD68 antigen. Moreover, PV-90 cells constitutively express the c-fms proto-oncogene mRNA as the patient's blast cells did. Whereas a trisomy 11 (+11) was found in the patient's bone marrow cells, PV-90 cells had a normal karyotype initially, but at 4 months showed two different and independent chromosomal abnormalities: 90, XX, -Y, -Y, t(9;16) (q11;p13), and 90, XX, -Y, -Y, t(17;18) (p13;q21), the latter possibly involving the p53 (17,p13) and bcl-2 (18, q21) proto-oncogenes. The early development of these chromosomal aberrations is consistent with a genetic instability of PV-90 cells. Expression of bi-lineage markers and genetic instability may suggest that PV-90 cells originated from transformation of a myelodysplastic progenitor cell capable of both myeloid and B-cell differentiation. The PV-90 cell line might be useful in a number of studies, including the possible role of c-fms in cell differentiation, pathogenetic mechanisms of human preleukaemia and lineage promiscuity in acute leukaemia."@en ; prodottidellaricerca:prodottoDi istituto:CDS040 ; pubblicazioni:autoreCNR unitaDiPersonaleInterno:MATRICOLA5292 . ns10:ID293206 pubblicazioni:rivistaDi prodotto:ID324541 .